Abstract
References
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- (2) Liang AL, Qian HL, Zhang TT, et al. Bifunctional fused polypeptide inhibits the growth and metastasis of breast cancer. Drug Des Devel Ther. 2015; 9:5671-86. (3) Miao J, Du YZ, Yuan H, Zhang XG, Hu FQ. Drug resistance reversal activity of anticancer drug loaded solid lipid nanoparticles in multi-drug resistant cancer cells. Colloids Surf B Biointerfaces. 2013, 110: 74-80.
- (4) Cabeza L, Ortiz R, Arias JL, et al. Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles. Int J Nanomedicine. 2015;13(10):1291-306.
- (5) Cancer Genome Atlas, Network Comprehensive molecular portraits of human breast tumours. Nature 490 (7418): 61-70.2012 DOI: 10.1038/nature11412.
- (6) Shargh VH, Hondermarck H, Liang M. Antibody-targeted biodegradable nanoparticles for cancer therapy. Nanomedicine (Lond). 2016;11(1):63-79.
- (7) Alvarez RH, Valero V, Hortobagyi GN. Emerging targeted therapies for breast cancer. J Clin Oncol. 2010;28(20):3366-79.
- (8) Spridon D, Panaitescu L, Ursu D, Uglea CV. Synthesis and biocompatibility of maleic anhydride copolymers: 1. Maleic anhydride-vinyl acetate, maleic anhydride-methyl methacrylate and maleic anhydride-styrene. The Cambridge Polymer Conference: Partnership in Polymers, Cambridge, UK, 30 Sep-2 Oct 1996 Polym Int 1997; 43(2): 175-81.
- (9) Hoste K, Winne KD, Schacht E. Polymeric prodrug. Int J Pharm 2004; 277:119–31.
- (10) Karakus G, Zengin HB, Polat ZA, Yenidunya AF, Aydin S. Cytotoxicity of three maleic anhydride copolymers and common solvents used for polymer solvation. Polymer Bulletin. 2013; 70(5): 591–12.
- (11) Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 1990; 82(13):11-07.
- (12) Karakuş G, Malatyalı E, Zengin H, Değerli S. In vitro amoebicidal activity of poly(maleic anhydride-co-vinyl acetate) copolymer on Acanthamoeba spp. trophozoites and cysts. Basic Clin Sci 2013; 2: 1-14.
- (13) Chai F, Zhang L, Xiao X, Duan C, Huang Q, Fan C, Li J et al. Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells. Oncology Reports 2016; 36:1180-86.
Cytotoxic and apoptotic effects of poly (maleic anhydride-co-vinyl acetate) drug carrier copolymer on MCF-7 and MDA-MB-231 breast cancer cells
Abstract
In
recent years, copolymers are frequently used in many areas. The
biocompatibility of any copolymer should be examined for practical application.
One of these copolymers is Poly [(maleic anhydride) -co- (vinyl acetate)]
(MAVA), and the usage area of MAVA is quite limited. In this study, the
cytotoxic effect of MAVA on MDA-MB-231 and MCF-7 human breast cancer cells was
determined by MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium
bromide), and apoptotic cells are marked with DAPI staining. For this purpose,
MDA-MB-231 and MCF-7 human breast cancer cells were incubated with different
concentrations of MAVA (1, 10, 50, 80, 100, 200, 300, 500, 800, and 1000 μM)
for 24 h, 48 h, and 72 h. IC50 values
(concentration of the test compound to achieve 50% of cell death ) of MAVA in MDA-MB-231 and MCF-7 human breast
cancer cells were determined (n=9). According to our results, it was observed
that MDA-MB-231 breast cancer cells increased 24 h and 48 h after MAVA application
compared to the control group and no significant change was observed after 72 h
MAVA application. In MCF-7 cells, a significant decrease was observed 24 h and
48 h after MAVA application compared to control, and no significant changes was
observed after 72 hours similar to MDA-MB-231. DAPI staining showed that more
apoptotic cells were found among the MCF-7 cells decreased, MDA-MB-231 cells
after MAVA application at 24 h, 48 h, 72 h. Despite the viability of MCF-7
cells decreased, MDA-MB-231 cell viability increased at 24 h and 48 h after
MAVA application. therefore it could be suggested that MAVA showed a selective
cytotoxic effect between MDA-MB-231 and MCF-7 breast cancer cells.
References
- (1) Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries CA. Cancer J Clin 2018; 68(6): 394-424.
- (2) Liang AL, Qian HL, Zhang TT, et al. Bifunctional fused polypeptide inhibits the growth and metastasis of breast cancer. Drug Des Devel Ther. 2015; 9:5671-86. (3) Miao J, Du YZ, Yuan H, Zhang XG, Hu FQ. Drug resistance reversal activity of anticancer drug loaded solid lipid nanoparticles in multi-drug resistant cancer cells. Colloids Surf B Biointerfaces. 2013, 110: 74-80.
- (4) Cabeza L, Ortiz R, Arias JL, et al. Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles. Int J Nanomedicine. 2015;13(10):1291-306.
- (5) Cancer Genome Atlas, Network Comprehensive molecular portraits of human breast tumours. Nature 490 (7418): 61-70.2012 DOI: 10.1038/nature11412.
- (6) Shargh VH, Hondermarck H, Liang M. Antibody-targeted biodegradable nanoparticles for cancer therapy. Nanomedicine (Lond). 2016;11(1):63-79.
- (7) Alvarez RH, Valero V, Hortobagyi GN. Emerging targeted therapies for breast cancer. J Clin Oncol. 2010;28(20):3366-79.
- (8) Spridon D, Panaitescu L, Ursu D, Uglea CV. Synthesis and biocompatibility of maleic anhydride copolymers: 1. Maleic anhydride-vinyl acetate, maleic anhydride-methyl methacrylate and maleic anhydride-styrene. The Cambridge Polymer Conference: Partnership in Polymers, Cambridge, UK, 30 Sep-2 Oct 1996 Polym Int 1997; 43(2): 175-81.
- (9) Hoste K, Winne KD, Schacht E. Polymeric prodrug. Int J Pharm 2004; 277:119–31.
- (10) Karakus G, Zengin HB, Polat ZA, Yenidunya AF, Aydin S. Cytotoxicity of three maleic anhydride copolymers and common solvents used for polymer solvation. Polymer Bulletin. 2013; 70(5): 591–12.
- (11) Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 1990; 82(13):11-07.
- (12) Karakuş G, Malatyalı E, Zengin H, Değerli S. In vitro amoebicidal activity of poly(maleic anhydride-co-vinyl acetate) copolymer on Acanthamoeba spp. trophozoites and cysts. Basic Clin Sci 2013; 2: 1-14.
- (13) Chai F, Zhang L, Xiao X, Duan C, Huang Q, Fan C, Li J et al. Cucurbitacin B reverses multidrug resistance by targeting CIP2A to reactivate protein phosphatase 2A in MCF-7/adriamycin cells. Oncology Reports 2016; 36:1180-86.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Basic Science Research Articles |
| Authors | |
| Publication Date | September 30, 2019 |
| Acceptance Date | September 27, 2019 |
| Published in Issue | Year 2019Volume: 41 Issue: 3 |