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Evaluation of in vitro anticancer activity of wheat germ oil in MCF-7 breast cancer cell lines

Yıl 2019, Cilt: 41 Sayı: 3, 500 - 505, 30.09.2019

Mustafa Ergül , Adamu Bekele Hatice Terzi , Ahmet Altun

https://doi.org/10.7197/cmj.vi.578764

Öz

Cancer
is the main cause of death and morbidity worldwide. Among females, breast
cancer is the most commonly diagnosed and the leading cause of cancer-related
death. Despite an improvement in the treatment of cancer in the latest years,
metastatic breast cancer treatment success is not at a desirable level. Plants
have huge potential to endow us with noble drugs and current drugs of plant
origin are important part of cancer chemotherapy. The aim of this study was to
evaluate the antiproliferative activity of wheat germ oil in MCF-7 breast cancer
cell line. Antiproliferative
activity and apoptotic effect of wheat germ oil were examined using the XTT
and flow cytometry assay, respectively. According to the XTT results, at concentrations
greater than 0.5 mg/mL, wheat germ oil displayed significant antiproliferative
activity in MCF-7 cells in a dose-dependent manner. The Annexin V binding assay
results also demonstrated that the IC50 concentration of wheat germ oil
increased early and late apoptotic populations (p < 0.05). Though further
studies are required to
specifically identify compounds involved in the anticancer activity of wheat
germ oil, our findings demonstrate that wheat germ oil inhibits proliferation
of MCF-7 cells and its action is dose-dependent.

Anahtar Kelimeler

Cancer apoptosis MCF-7

Kaynakça

  • Bray F, Jemal A, Grey N, et al. Global cancer transitions according to the Human Development Index (2008–2030): a population-based study. The lancet oncology. 2012;13(8):790-801.
  • Xu J, Murphy SL, Kochanek KD, et al. Deaths: Final data for 2016. 2018.
  • Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians. 2018;68(6):394-424.
  • Cardoso F, Costa A, Norton L, et al. 1st International consensus guidelines for advanced breast cancer (ABC 1). The Breast. 2012;21(3):242-52.
  • Chakraborty S, Rahman T. The difficulties in cancer treatment. ecancermedicalscience. 2012;6.
  • Cragg GM, Pezzuto JM. Natural products as a vital source for the discovery of cancer chemotherapeutic and chemopreventive agents. Medical Principles and Practice. 2016;25(Suppl. 2):41-59.
  • Demain AL, Vaishnav P. Natural products for cancer chemotherapy. Microbial biotechnology. 2011;4(6):687-99.
  • Sjövall O, Virtalaine T, Lapveteläinen A, Kallio H. Development of rancidity in wheat germ analyzed by headspace gas chromatography and sensory analysis. Journal of agricultural and food chemistry. 2000;48(8):3522-7.
  • Haddow A, Russell H. The influence of wheat germ oil in the diet on the induction of tumours in mice. The American Journal of Cancer. 1937;29(2):363-6.
  • Chamras H, Barsky SH, Ardashian A, et al. Novel interactions of vitamin E and estrogen in breast cancer. Nutrition and cancer. 2005;52(1):43-8.
  • Figueroa D, Asaduzzaman M, Young F. Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines In Vitro. BioMed research international. 2019;2019.
  • Fernandes RS, Silva JO, Seabra HA, et al. α-Tocopherol succinate loaded nano-structed lipid carriers improves antitumor activity of doxorubicin in breast cancer models in vivo. Biomedicine & Pharmacotherapy. 2018;103:1348-54.
  • Khallouki F, de Medina P, Caze-Subra S, et al. Molecular and biochemical analysis of the estrogenic and proliferative properties of vitamin E compounds. Frontiers in oncology. 2016;5:287.
  • Diao Q, Zhang J, Zhao T, et al. Vitamin E promotes breast cancer cell proliferation by reducing ROS production and p53 expression. Eur Rev Med Pharmacol Sci. 2016;20(12):2710-7.
  • Malafa MP, Neitzel LT. Vitamin E succinate promotes breast cancer tumor dormancy. Journal of Surgical Research. 2000;93(1):163-70.
  • Sayin VI, Ibrahim MX, Larsson E, et al. Antioxidants accelerate lung cancer progression in mice. Science translational medicine. 2014;6(221):221ra15-ra15.
  • ŞELECİ DA, GÜMÜŞ ZP, Yavuz M, et al. A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil. Turkish Journal of Chemistry. 2015;39(4):801-12.
  • Imir NG, Aydemir E, Şimşek E. Mechanism of the anti-angiogenic effect of avemar on tumor cells. Oncology letters. 2018;15(2):2673-8.
  • Fajka-Boja R, Hidvegi M, Shoenfeld Y, et al. Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines. International Journal of Oncology. 2002;20(3):563-70.
  • Judson PL, Al Sawah E, Marchion DC, et al. Characterizing the efficacy of fermented wheat germ extract against ovarian cancer and defining the genomic basis of its activity. International Journal of Gynecologic Cancer. 2012;22(6):960-7.
  • Yang M-D, Chang W-S, Tsai C-W, et al. Inhibitory effects of AVEMAR on proliferation and metastasis of oral cancer cells. Nutrition and cancer. 2016;68(3):473-80.

Yıl 2019, Cilt: 41 Sayı: 3, 500 - 505, 30.09.2019

Mustafa Ergül , Adamu Bekele Hatice Terzi , Ahmet Altun

https://doi.org/10.7197/cmj.vi.578764

Öz


Kaynakça

  • Bray F, Jemal A, Grey N, et al. Global cancer transitions according to the Human Development Index (2008–2030): a population-based study. The lancet oncology. 2012;13(8):790-801.
  • Xu J, Murphy SL, Kochanek KD, et al. Deaths: Final data for 2016. 2018.
  • Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians. 2018;68(6):394-424.
  • Cardoso F, Costa A, Norton L, et al. 1st International consensus guidelines for advanced breast cancer (ABC 1). The Breast. 2012;21(3):242-52.
  • Chakraborty S, Rahman T. The difficulties in cancer treatment. ecancermedicalscience. 2012;6.
  • Cragg GM, Pezzuto JM. Natural products as a vital source for the discovery of cancer chemotherapeutic and chemopreventive agents. Medical Principles and Practice. 2016;25(Suppl. 2):41-59.
  • Demain AL, Vaishnav P. Natural products for cancer chemotherapy. Microbial biotechnology. 2011;4(6):687-99.
  • Sjövall O, Virtalaine T, Lapveteläinen A, Kallio H. Development of rancidity in wheat germ analyzed by headspace gas chromatography and sensory analysis. Journal of agricultural and food chemistry. 2000;48(8):3522-7.
  • Haddow A, Russell H. The influence of wheat germ oil in the diet on the induction of tumours in mice. The American Journal of Cancer. 1937;29(2):363-6.
  • Chamras H, Barsky SH, Ardashian A, et al. Novel interactions of vitamin E and estrogen in breast cancer. Nutrition and cancer. 2005;52(1):43-8.
  • Figueroa D, Asaduzzaman M, Young F. Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines In Vitro. BioMed research international. 2019;2019.
  • Fernandes RS, Silva JO, Seabra HA, et al. α-Tocopherol succinate loaded nano-structed lipid carriers improves antitumor activity of doxorubicin in breast cancer models in vivo. Biomedicine & Pharmacotherapy. 2018;103:1348-54.
  • Khallouki F, de Medina P, Caze-Subra S, et al. Molecular and biochemical analysis of the estrogenic and proliferative properties of vitamin E compounds. Frontiers in oncology. 2016;5:287.
  • Diao Q, Zhang J, Zhao T, et al. Vitamin E promotes breast cancer cell proliferation by reducing ROS production and p53 expression. Eur Rev Med Pharmacol Sci. 2016;20(12):2710-7.
  • Malafa MP, Neitzel LT. Vitamin E succinate promotes breast cancer tumor dormancy. Journal of Surgical Research. 2000;93(1):163-70.
  • Sayin VI, Ibrahim MX, Larsson E, et al. Antioxidants accelerate lung cancer progression in mice. Science translational medicine. 2014;6(221):221ra15-ra15.
  • ŞELECİ DA, GÜMÜŞ ZP, Yavuz M, et al. A case study on in vitro investigations of the potent biological activities of wheat germ and black cumin seed oil. Turkish Journal of Chemistry. 2015;39(4):801-12.
  • Imir NG, Aydemir E, Şimşek E. Mechanism of the anti-angiogenic effect of avemar on tumor cells. Oncology letters. 2018;15(2):2673-8.
  • Fajka-Boja R, Hidvegi M, Shoenfeld Y, et al. Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines. International Journal of Oncology. 2002;20(3):563-70.
  • Judson PL, Al Sawah E, Marchion DC, et al. Characterizing the efficacy of fermented wheat germ extract against ovarian cancer and defining the genomic basis of its activity. International Journal of Gynecologic Cancer. 2012;22(6):960-7.
  • Yang M-D, Chang W-S, Tsai C-W, et al. Inhibitory effects of AVEMAR on proliferation and metastasis of oral cancer cells. Nutrition and cancer. 2016;68(3):473-80.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Medical Science Research Makaleler
Yazarlar

Mustafa Ergül 0000-0003-4303-2996

Adamu Bekele

Hatice Terzi

Ahmet Altun 0000-0003-2056-8683

Yayımlanma Tarihi 30 Eylül 2019
Kabul Tarihi 30 Eylül 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 41 Sayı: 3

Kaynak Göster

AMA Ergül M, Bekele A, Terzi H, Altun A. Evaluation of in vitro anticancer activity of wheat germ oil in MCF-7 breast cancer cell lines. CMJ. Eylül 2019;41(3):500-505. doi:10.7197/cmj.vi.578764