LITHIUM HAS NEUROPROTECTIVE EFFECT ON NEUROBLASTOMA CELL LINE IN LOW DOSAGES

Şefik Güran; Zehra Çoban; Hülya Gündeşli; Özgür Kılıçarslan

doi:10.7197/cmj.1172075

EN

LITHIUM HAS NEUROPROTECTIVE EFFECT ON NEUROBLASTOMA CELL LINE IN LOW DOSAGES

Abstract

Lithium (Li) was presented as a protective agent in neuron degeneration which is an important process in neurodegenerative diseases. The aim of this study was to determine the effect of certain amounts of Li on neuroblastoma cells by considering several specific genes which act on neuroprotection. Li solutions were prepared as 1 μM, 15 μM (low dosage), 30 μM and 45 µM (high dosage) concentrations then applied to neuroblastoma cell line. XTT and trypan blue assays were performed to determine the cell proliferation and viability, respectively. mRNA expression levels of NES, BDNF, GRIN2A, LRRK2, PRKN, and SNCA were detected by quantitative real time polymerase chain reaction (qRT-PCR). Cell viability detected as significantly increased in cells treated with low dosage Li however, it was significantly decreased in high dosage applied cells compared to untreated control. In addition, cell proliferation ratios were significantly decreased in high dosage applied cells compared to control. It was demonstrated that mRNA expression levels of several genes (NES, LRRK2, PRKN) were significantly upregulated. Regarding to BDNF, expression of the gene was significantly upregulated in the cells only treated with very low amount of Li. However, no significant data could be obtained for GRIN2A. Furthermore, mRNA expression level of SNCA was determined as significantly downregulated compared to control. Statistically significant expression of NES, LRRK2, PRKN, BDNF and SNCA genes due to the variable Li concentrations applied to cells suggests that Li acts on transcriptional regulation of certain genes associated with neuronal survival. These findings support that dose dependent Li treatment might have a protective effect for neurodegenerative diseases.

Keywords

Destekleyen Kurum

TC GENELKURMAY BAŞKANLIĞI GÜLHANE ASKERİ TIP AKADEMİSİ KOMUTANLIĞI

Proje Numarası

AR-2015/22

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Şefik Güran ^*
0000-0002-1398-530X
Türkiye

Zehra Çoban
0000-0001-6397-9138
Türkiye

Hülya Gündeşli
0000-0002-1638-9087
Türkiye

Özgür Kılıçarslan
0000-0001-6106-708X
Türkiye

Yayımlanma Tarihi

31 Mart 2023

Gönderilme Tarihi

7 Eylül 2022

Kabul Tarihi

26 Mart 2023

Yayımlandığı Sayı

Yıl 1970 Cilt: 45 Sayı: 1

DOI

https://doi.org/10.7197/cmj.1172075

IZ

https://izlik.org/JA37RL64FM

Kaynak Göster

RIS / Bibtex

AMA

1.Güran Ş, Çoban Z, Gündeşli H, Kılıçarslan Ö. LITHIUM HAS NEUROPROTECTIVE EFFECT ON NEUROBLASTOMA CELL LINE IN LOW DOSAGES. CMJ. 2023;45(1):17-24. doi:10.7197/cmj.1172075