RELATIONSHIP BETWEEN SHELTERIN PROTEINS AND AGMATINE IN URINARY TRACT INFECTIONS

Mustafa Doğan Bedir; Sevtap Bakır; Aynur Engin

doi:10.7197/cmj.1294625

EN TR

RELATIONSHIP BETWEEN SHELTERIN PROTEINS AND AGMATINE IN URINARY TRACT INFECTIONS

Abstract

Abstract: The presence of microorganisms in the kidney, collecting system, or bladder with pyuria and clinical symptoms is called Urinary Tract Infection (UTI). UTI is one of the most common diseases in both nosocomial and community-acquired infections and affects millions of people every year. The clinical types of UTI range from cystitis to sepsis. In this study, we aimed to contribute to the literature by comparing the levels of shelterin proteins and agmatine in the serum of patients diagnosed with cystitis, one of the types of UTI, and healthy volunteers without any known medical disorder, and to bring new approaches to issues such as clinical severity, response to treatment, and pathogenesis of the disease. Methods: In this study, 30 individuals diagnosed with cystitis from UTIs and 30 individuals without any systemic disease participated. Serum agmatine levels were measured by a fluorescence detector using the ultra-high-performance liquid chromatography method (UHPLC). Serum shelterin proteins levels were measured using the ELISA method. Results: When the patients and healthy controls were compared, serum Telomeric Repeat Binding Factor2 (TRF2) levels of the patients were found to be lower than the controls; serum agmatine levels were found to be higher than the controls and statistically significant (p<0.05). Other shelterin protein levels were not statistically different between patients and controls (p>0.05). Conclusion: In conclusion, the statistically significant difference in serum TRF2 and agmatine levels between the groups may be associated with oxidative stress. It is thought that the shortening of telomere lengths may be associated with decreased TRF2 levels.

Keywords

ÜRİNER SİSTEM ENFEKSİYONLARINDA SHELTERİN PROTEİNLERİ VE AGMATİN İLİŞKİSİ

Öz

Özet: Piyüri ve klinik semptomlarla birlikte böbrekte, toplayıcı sistemde veya mesanede mikroorganizmanın bulunması, Üriner Sistem Enfeksiyonu (ÜSE) olarak adlandırılır. ÜSE, hem nozokomyal enfeksiyonlar hem de toplumdan kazanılmış enfeksiyonlarda en sık rastlanan hastalıklar arasında yer almaktadır ve her yıl milyonlarca insanı etkilemektedir. ÜSE’nin klinik tipleri, sistitten sepsise kadar değişmektedir. Bu çalışmada, ÜSE türlerinden biri olan sistit tanısı almış hastalar ile bilinen herhangi bir tıbbi bozukluğu olmayan sağlıklı gönüllü kişilerin serumlarında, shelterin proteinleri ve agmatin düzeylerinin karşılaştırılarak hastalığın klinik ciddiyeti, tedaviye yanıt takibi, patogenezi gibi hususlara yeni yaklaşımlar getirilmesi ve literarüre katkı sağlanması amaçlanmıştır. Yöntemler: Bu çalışmada, ÜSE’lerden sistit tanısı almış 30 birey ve herhangi bir sistemik hastalığı olmayan 30 birey katılmıştır. Serum agmatin seviyeleri ultra yüksek performanslı sıvı kromatografi yöntemi (UHPLC) kullanılarak floresans dedektörle ölçülmüştür. Serum shelterin protein düzeyleri ise ELISA yöntemi kullanılarak ölçülmüştür. Bulgular: Hastalar ile sağlıklı kontroller karşılaştırıldığında, hastaların serum Telomerik Tekrar Bağlanma Faktörü2 (TRF2) düzeyleri kontrollere göre düşük; serum agmatin düzeyleri kontrolllere göre yüksek bulunmuştur ve istatistiksel olarak anlamlıdır (p<0,05). Diğer shelterin protein düzeyleri ise hasta ve kontrol arasında istatistiksel farklı hesaplanmamıştır (p>0,05). Sonuç: Sonuç olarak, serum TRF2 ve agmatin düzeylerinin gruplar arasında istatistiksel olarak anlamlı farklılık göstermesi oksidatif stres ile ilişkilendirilebilir. TRF2 düzeylerindeki azalma ile telomer uzunluklarında kısalma olabileceği düşünülmektedir.

Anahtar Kelimeler

Destekleyen Kurum

SİVAS CUMHURİYET ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ (CÜBAP)

Proje Numarası

T-812

Teşekkür

Bu çalışma Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri Komisyonu tarafından T-812 numaralı doktora tez projesi kapsamında maddi olarak desteklenmiştir

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Mustafa Doğan Bedir ^*
0000-0002-2628-0739
Türkiye

Sevtap Bakır
0000-0003-1956-0844
Türkiye

Aynur Engin
0000-0002-8533-8793
Türkiye

Yayımlanma Tarihi

31 Aralık 2023

Gönderilme Tarihi

9 Mayıs 2023

Kabul Tarihi

14 Aralık 2023

Yayımlandığı Sayı

Yıl 1970 Cilt: 45 Sayı: 4

DOI

https://doi.org/10.7197/cmj.1294625

IZ

https://izlik.org/JA33NS85YB

Kaynak Göster

RIS / Bibtex

AMA

1.Bedir MD, Bakır S, Engin A. RELATIONSHIP BETWEEN SHELTERIN PROTEINS AND AGMATINE IN URINARY TRACT INFECTIONS. CMJ. 2023;45(4):26-35. doi:10.7197/cmj.1294625