Monosodium glutamate below the neurotoxic doses has no cytotoxic effect on mouse mesenchymal stem cells

Sinem Dal; Sümeyye Arslan; Nejat Kaan Nurol; Tutku Göktepe; Zehra Dilşad Çoban; Ertan Altaylı; Şefik Güran

doi:10.7197/223.v39i31705.347449

EN TR

Monosodium glutamate below the neurotoxic doses has no cytotoxic effect on mouse mesenchymal stem cells

Abstract

Objective: Monosodium glutamate appear as a food additive that listed in the group of flavor enhancers is continuously used in various types of foods. Monosodium glutamate is the sodium salt of glutamic acid, one of the most abundant naturally-occurring non-essential amino acids. U.S. Food and Drug Administration reported that monosodium glutamate is safe for use in food. However, the excessive use of monosodium glutamate causes dizziness, nausea and vomiting. Due to reports, it causes obesity. In this study it was aimed to find out the cytotoxicity of monosodium glutamate and the role on the selected genes, which have role on obesity.

Method: In our study, mice were used which are known to be the most sensitive in terms of toxicity. The cytotoxic effects of monosodium glutamate on mouse mesenchymal stem cells at monosodium glutamate doses below the blood dose (100-130 µmol/dl) that reported as the neuronal damage threshold in mice were studied. Furthermore leptin-lep and ghrelin / obestatin in prepropeptide-GHRL gene expressions in order to find out the role of monosodium glutamate in obesity were analyzed.

Results: Monosodium glutamate below the toxic dose does not have a cytotoxic effect on mouse mesenchymal stem cells. Also no expression change in applied monosodium glutamate doses was observed in genes which are known to be associated with obesity.

Conclusions: Our results support that monosodium glutamate has no toxic effect on stem cells in uses in certain doses.

Keywords

Monosodium glutamate,mouse mesenchymal stem cells,leptin gene,ghrelin/obestatin prepropeptide gene

Nörotoksik dozun altındaki monosodyum glutamatın fare mezenkimal kök hücreler üzerinde sitotoksik etkisi yoktur

Öz

Amaç: Monosodyum glutamat lezzet arttırıcılar grubunda gıda katkı maddesi olarak çeşitli yiyeceklerde kullanılmaktadır. Monosodyum glutamat glutamik asidin sodyum tuzudur ve doğal yollarla en fazla oluşan esansiyel olmayan aminoasitlerden biridir. Amerikan Gıda Ve İlaç Teşkilatı (U.S. Food and Drug Administration) monosodyum glutamatın gıdada kullanımının güvenli olduğunu bildirmiştir. Ancak aşırı kullanımında baş dönmesi, bulantı kusma yapmaktadır. Makalelerde obeziteye neden olduğu bildirilmektedir. Bu çalışmada, monosodyum glutamatın sitotoksik ve obezitede rolü olan seçilmiş bazı genlere olan etkilerinin bulunması amaçlanmıştır.

Yöntem: Çalışmamıza toksisite yönünden en hassas organizma olduğu bilinen fareler alınmıştır. Monosodyum glutamatın fare mezenkimal hücreler üzerine olan sitotoksik etkisi farelerde nöronal hasar oluşturduğu bildirilen kan dozunun (100-130 µmol/dl) altındaki dozlar kullanılarak çalışılmıştır. Ayrıca monosodyum glutamatın obezite üzerine olan etkisini bulmak için “leptin-lep” ve “Ghrelin/Obestatin Prepropeptide-GHRL” genlerine ait ekspresyon değişikliklerine bakılmıştır.

Bulgular: Monosodyum glutamatın toksik dozun altında fare mezenkimal kök hücre üzerinde bir sitotoksik etkisi olmadığı bulunmuştur. Uygulanan monosodyum glulatamat dozlarında hücrede obezite ile ilgili olduğu bilinen genlerin ekspresyonlarını değiştirmediği saptanmıştır.

Sonuç: Sonuçlarımız monosodyum glutamatın gıdada belli dozlarda kullanımının kök hücreler üzerine toksik bir etkisi olmadığını destekler niteliktedir.

Anahtar Kelimeler

Monosodyum glutamat,fare mezenkimal kök hücresi,leptin geni,ghrelin/obestatin prepropeptide geni

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Sinem Dal

Sümeyye Arslan

Nejat Kaan Nurol

Tutku Göktepe

Zehra Dilşad Çoban

Ertan Altaylı

Şefik Güran

Yayımlanma Tarihi

19 Eylül 2017

Gönderilme Tarihi

19 Nisan 2017

Kabul Tarihi

25 Temmuz 2017

Yayımlandığı Sayı

Yıl 1970 Cilt: 39 Sayı: 3

DOI

https://doi.org/10.7197/223.v39i31705.347449

IZ

https://izlik.org/JA86HX74AE

Kaynak Göster

RIS / Bibtex

AMA

1.Dal S, Arslan S, Nurol NK, vd. Monosodium glutamate below the neurotoxic doses has no cytotoxic effect on mouse mesenchymal stem cells. CMJ. 2017;39(3):525-530. doi:10.7197/223.v39i31705.347449

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