Clinical symptoms of rat acute peritonitis model and study over effects of oral strontium ranelate administration for the treatment

Sinan Soylu; Atilla Kurt; Zeynep Deniz Şahin İnan; Hüsnü Çağrı Genç; Hakkı Coşkun; Musa Serin

doi:10.7197/223.vi.356042

TR EN

Rat, akut peritonit modelinde klinik semptomlar ve hastalığın tedavisine oral strontium ranilat uygulamalarının etkilerinin araştırılması

Öz

Amaç: Cerrahi ve yoğun bakım tedavilerinde ki gelişmelere rağmen sepsis ve sistemik inflamatuar cevap sendromu(SIRS) yetişkin yoğun bakım ünitelerinde ensık ölüm sebeplerindendir. Bu sebeple peritonit ve sepsis için deney hayvanı modelinde stronsiyum ranilat’ın iyileşme üzerine etkisini biyokimyasal ve histopatolojik olarak ortaya koymayı amaçladık.

Yöntem: Wistar-Albino cinsi 40 adet dişi sıçan kullanıldı. Sıçanlar; kontrol, sham[çekum ligasyon perforasyon(ÇLP)], ÇLP + Stronsiyum(S) ve S + ÇLP + S olmak üzere 4 gruba ayrıldı. Sıçanlar 7. günde ötanazi uygulandı. Peritoneal inflamasyon evrelemesi yapıldı. Patoloji için ileum rezeksiyonu yapıldı, biokimyasal inceleme için kan örnekleri alınarak IL-1, IL-6, IL- 10 ve TNF-alpha, bakıldı.

Bulgular: Peritoneal inflamasyon skoru en düşük kontrol grubunda, en yüksek CLP grubunda bulundu. ÇLP+S grubunda ÇLP grubuna göre iyileşme, S+ÇLP+S grubunda ise ÇLP+S grubuna göre daha fazla iyileşme vardı. Histopatolojik değerlendirmeye göre de incebarsak hasarı en düşük kontrol grubunda, en yüksek ÇLP grubunda bulundu. ÇLP+S grubunda ÇLP grubuna göre daha hafif hasar, S+ÇLP+S grubunda ise ÇLP+S grubuna göre daha hafif hasar vardı. TNF-α, IL-1, IL-6 ve Il-10 değerleri karşılaştırıldığında fark yoktu.

Sonuç: Stronsiyum ranilat sepsis riski olan ve sepsis gelişmiş hastalarda proflaktik tedavi ile mortalite ve morbiditeyi azaltabilir.

Anahtar Kelimeler

Peritonit,sepsis,çekum ligasyon perforasyon,stronsiyum

Clinical symptoms of rat acute peritonitis model and study over effects of oral strontium ranelate administration for the treatment

Abstract

Objective: Despite the improvements in the treatments of surgical and intensive care, sepsis and systemic inflammatory response syndrome are the most common cause of mortality. Therefore in the experimental animal model, we aimed to reveal biochemical and histopathologic effects of strontium ranelate on healing in experimental animal model for peritonitis and sepsis.

Method: 20 female Wistar-Albino rats were used.Rats were seperated into 4 groups; control, sham [ceacum ligation perforation (CLP)], CLP + Strontium (S) (CLP+S) and S+CLP+S. Rats were euthanised on 7th day. Peritoneal inflammation staging and ileum resection was performed. Blood samples were taken for biochemical screening and IL-1, IL-6, IL-10 and TNF-alpha tests were evaluated.

Results: Peritoneal inflammation score was the highest at CLP group and the lowest at control group. There was healing in CLP+S group compared to CLP group, and more healing in S+CLP+S group compared to CLP+S group. According to histopathological evaluation, ileum injury was the lowest in the control group and the highest in CLP group. Less injury was found in CLP+S group compared to CLP group, and less injury was found in S+CLP+S group compared to CLP+S group. No difference was found when TNF-alpha, IL-1, IL-6 and IL-10 values were compared.

Conclusions: Strontium ranelate may reduce mortality and morbidity by prophylactic treatment in patients at risk of sepsis and patients with sepsis.

Keywords

Peritonitis,sepsis,ceacum ligation perforation,strontium

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Sinan Soylu
Türkiye

Atilla Kurt
Türkiye

Zeynep Deniz Şahin İnan

Hüsnü Çağrı Genç

Hakkı Coşkun

Musa Serin

Yayımlanma Tarihi

20 Mart 2018

Gönderilme Tarihi

18 Kasım 2017

Kabul Tarihi

15 Mart 2018

Yayımlandığı Sayı

Yıl 2018 Cilt: 40 Sayı: 1

DOI

https://doi.org/10.7197/223.vi.356042

IZ

https://izlik.org/JA53LR63BY

Kaynak Göster

RIS / Bibtex

AMA

1.Soylu S, Kurt A, İnan ZDŞ, Genç HÇ, Coşkun H, Serin M. Clinical symptoms of rat acute peritonitis model and study over effects of oral strontium ranelate administration for the treatment. CMJ. 2018;40(1):71-77. doi:10.7197/223.vi.356042