Araştırma Makalesi
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Yıl 2020, , 86 - 93, 20.05.2020
https://doi.org/10.7197/cmj.vi.700866

Öz

Destekleyen Kurum

Marmara Üniversitesi Bilimsel Araştırmalar Projesi

Proje Numarası

BAPKO, SAG-C-DRP-131216-0537

Kaynakça

  • REFERENCES Reference 1. Rana P, Ganarajan G, Kothiyal P. Review on preparation and properties hydrogel formulation. WJPPS. 2015; 4 (12): 1069-1087.
  • Reference 2. Pal K, Banthia AK, Majumdar DK. Polymeric Hydrogels: Characterization and Biomedical Applications. Des Monomers Polym. 2009; 12: 197- 220.
  • Reference 3. Mohamed RR, Abu Elella MH, Sabaa MW. Synthesis, characterization and applications of N-quaternized chitosan/poly (vinyl alcohol) hydrogels. Int J Biol Macromol. 2015; 80: 149–161.
  • Reference 4. Vino AB, Ramasamy P, Shanmugam V, Shanmugam A. Extraction, characterization and in vitro antioxidative potential of chitosan and sulfated chitosan from Cuttlebone of Sepia aculeata Orbigny, 1848. Asian. Pac. J. Trop. Biomed. 2012; 2: 334–S341.
  • Reference 5. Mansur HS, Sadahira CM, Souza AN, Mansur AAP. FTIR spectroscopy characterization of poly (vinyl alcohol) hydrogel with different hydrolysis degree and chemically crosslinked with glutaraldehyde. Mater Sci Eng. 2008; 28: 539–548.
  • Reference 6. Dogan S, Demirer S, Kepenekci I, Erkek B, Kiziltay A, Hasirci N, et al. Epidermal growth factor-containing wound closure enhances wound healing in non-diabetic and diabetic rats. Int Wound J. 2009; 6: 107-115.
  • Reference 7. Hardwicke J, Schmaljohann D, Boyce D, Thomas D. Epidermal Growth Factor Therapy and Wound Healing - Past, Present and Future Perspectives. Surgeon. 2008; 6(3): 172-177.
  • Reference 8. Jevsevar S, Kunstelj M, Porekar VG. PEGylation of therapeutic proteins. Biotechnol. J. 2010; 5: 113-128.
  • Reference 9. Lee H, Jang IH, Ryu SH, Park TG. N-Terminal Site-Specific Mono-PEGylation of Epidermal Growth Factor. Pharm Res. 2003; 20(5): 818-825.
  • Reference 10. Sezer AD, Cevher E, Hatipoğlu F, Oğurtan Z, Baş AL, Akbuğa J. Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits. Biol Pharm Bull. 2008; 31(12): 2326-2333.
  • Reference 11. Arranja A, Schroder AP, Schmutz M, Waton G, Schosseler F, Mendes E. Cytotoxicity and internalization of Pluronic micelles stabilized by core cross-linking. J Control Release. 2014; 196:87-95.
  • Reference 12. Wolf NB, Küchler S, Radowski MR, Blaschke T, Kramer KD, Weindi G, et al. Influences of opioids and nanoparticles on in vitro wound healing models. Eur J Pharm Biopharm. 2009; 73: 34-42.
  • Reference 13. Song C, Yu H, Zhang M, Yang Y, Zhang G. Physicochemical properties and antioxidant activity of chitosan from the blowfly Chrysomya megacephala larvae. Int. J. Biol. Macromol. 2013; 60: 347–354.

Synthesis of crosslinked PVA/Chitosan hydrogels loaded EGF and evaluation in vitro characterization

Yıl 2020, , 86 - 93, 20.05.2020
https://doi.org/10.7197/cmj.vi.700866

Öz

Objective: The aim of this study was to prepare different hydrogels using chitosan and PVA polymers and to evaluate the cytotoxicity of hydrogels through cell culture studies. In addition, FTIR spectrum measurements were aimed to determine the specific chemical groups and bonds in the structures of hydrogels and materials.
Method: In this study, different hydrogels were prepared and 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl tetrazolium bromide (MTT) test was performed to evaluate the toxicity of hydrogels on mouse fibroblast cell line (L-929). In addition, specific chemical groups of materials and hydrogels used in hydrogels were determined via FTIR spectrum study.
Results: According to the MTT cytotoxicity results B1 (94.20±1.30 %) and D2 (82.45±1.05 %) formulations showed the greatest and lowest L-929 cell viability respectively. According to the results of the FTIR absorption spectrum, the aliphatic C = O tension band of rh-EGF was observed at 1634 cm-1.
Conclusions: Results showed that chitosan-PVA hydrogels containing r-EGF types were observed to increase mouse fibroblast cell viability. In the FTIR spectrum results, spectral bands showing specific chemical groups of chitosan, PVA, PEGylation agents, rh-EGF and C2 formulation were obtained.

Proje Numarası

BAPKO, SAG-C-DRP-131216-0537

Kaynakça

  • REFERENCES Reference 1. Rana P, Ganarajan G, Kothiyal P. Review on preparation and properties hydrogel formulation. WJPPS. 2015; 4 (12): 1069-1087.
  • Reference 2. Pal K, Banthia AK, Majumdar DK. Polymeric Hydrogels: Characterization and Biomedical Applications. Des Monomers Polym. 2009; 12: 197- 220.
  • Reference 3. Mohamed RR, Abu Elella MH, Sabaa MW. Synthesis, characterization and applications of N-quaternized chitosan/poly (vinyl alcohol) hydrogels. Int J Biol Macromol. 2015; 80: 149–161.
  • Reference 4. Vino AB, Ramasamy P, Shanmugam V, Shanmugam A. Extraction, characterization and in vitro antioxidative potential of chitosan and sulfated chitosan from Cuttlebone of Sepia aculeata Orbigny, 1848. Asian. Pac. J. Trop. Biomed. 2012; 2: 334–S341.
  • Reference 5. Mansur HS, Sadahira CM, Souza AN, Mansur AAP. FTIR spectroscopy characterization of poly (vinyl alcohol) hydrogel with different hydrolysis degree and chemically crosslinked with glutaraldehyde. Mater Sci Eng. 2008; 28: 539–548.
  • Reference 6. Dogan S, Demirer S, Kepenekci I, Erkek B, Kiziltay A, Hasirci N, et al. Epidermal growth factor-containing wound closure enhances wound healing in non-diabetic and diabetic rats. Int Wound J. 2009; 6: 107-115.
  • Reference 7. Hardwicke J, Schmaljohann D, Boyce D, Thomas D. Epidermal Growth Factor Therapy and Wound Healing - Past, Present and Future Perspectives. Surgeon. 2008; 6(3): 172-177.
  • Reference 8. Jevsevar S, Kunstelj M, Porekar VG. PEGylation of therapeutic proteins. Biotechnol. J. 2010; 5: 113-128.
  • Reference 9. Lee H, Jang IH, Ryu SH, Park TG. N-Terminal Site-Specific Mono-PEGylation of Epidermal Growth Factor. Pharm Res. 2003; 20(5): 818-825.
  • Reference 10. Sezer AD, Cevher E, Hatipoğlu F, Oğurtan Z, Baş AL, Akbuğa J. Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits. Biol Pharm Bull. 2008; 31(12): 2326-2333.
  • Reference 11. Arranja A, Schroder AP, Schmutz M, Waton G, Schosseler F, Mendes E. Cytotoxicity and internalization of Pluronic micelles stabilized by core cross-linking. J Control Release. 2014; 196:87-95.
  • Reference 12. Wolf NB, Küchler S, Radowski MR, Blaschke T, Kramer KD, Weindi G, et al. Influences of opioids and nanoparticles on in vitro wound healing models. Eur J Pharm Biopharm. 2009; 73: 34-42.
  • Reference 13. Song C, Yu H, Zhang M, Yang Y, Zhang G. Physicochemical properties and antioxidant activity of chitosan from the blowfly Chrysomya megacephala larvae. Int. J. Biol. Macromol. 2013; 60: 347–354.
Toplam 13 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Basic Science Research Makaleler
Yazarlar

Murat Doğan 0000-0003-2794-0177

Ali Demir Sezer 0000-0002-2678-8903

Proje Numarası BAPKO, SAG-C-DRP-131216-0537
Yayımlanma Tarihi 20 Mayıs 2020
Kabul Tarihi 11 Mayıs 2020
Yayımlandığı Sayı Yıl 2020

Kaynak Göster

AMA Doğan M, Sezer AD. Synthesis of crosslinked PVA/Chitosan hydrogels loaded EGF and evaluation in vitro characterization. CMJ. Mayıs 2020;42(1):86-93. doi:10.7197/cmj.vi.700866