Objective: It was aimed to determine Cryptosporidium spp., which is thought to cause digestive system complaints
in primary school children, by Modified Acid-Fast (MAF) staining method and
Polymerase Chain Reaction (PCR).
Method: One hundred sixty four stool samples taken from children
were examined under light microscopy with MAF. Positive samples were fixed in
10% formalin and stored at + 4 °C until analysis. These samples were then
purified using the Sucrose Gradient method and, DNA isolation was performed
using the modified QIAamp DNA Mini Kit (Qiagen) protocol. In order to increase
sensitivity of the study, SSU rRNA gene region was amplified by PCR method.
Results: Microscopic examination showed that 89 of the 164 stool
samples (54,26%) were found to be positive for Cryptosporidium spp.oocysts. Eighty nine samples which were found
to be positive by microscopic examination and fixed in %10 formalin were
studied by PCR and 74 (45,12%) of them were positive.
Conclusions: Cryptosporidiosis, which is known to
cause severe infections, especially in immunocompromised individuals and
children, is transmitted by fecal-oral route from infected humans and animals,
and contaminated water and nutrients. Cryptosporidiosis, which is the result of
fecal pollution, causes a great risk for human health even with a low number of
oocysts due to the fact that oocysts are resistant to chlorine and they can
survive for a long time in the outer environment. It was observed that, primary
school children living in area of the study were under risk for
cryptosporidiosis.
Modified Acid-Fast (MAF) Polymerase Chain Reaction (PCR) Cryptosporidium spp.
Birincil Dil | İngilizce |
---|---|
Konular | Sağlık Kurumları Yönetimi |
Bölüm | Basic Science Research Makaleler |
Yazarlar | |
Yayımlanma Tarihi | 30 Haziran 2019 |
Kabul Tarihi | 16 Mayıs 2019 |
Yayımlandığı Sayı | Yıl 2019Cilt: 41 Sayı: 2 |