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Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)

Yıl 2021, Cilt: 43 Sayı: 1, 62 - 71, 31.03.2021

Hüsnü Genç Sinan Soylu , Deniz Şahin İnan , Atilla Kurt , Hakkı Çoşkun , Ali Yıldırır

https://doi.org/10.7197/cmj.904376

Öz

Objective: The basic aim in the treatment of second-degree burns is to prevent progressive cell death, and so treatments are directed at the zone of stasis. In this study, We were aimed to investigate the healing effects of using Mesenchymal Stem Cells (MSCs), Ovarian follicular fluid (OFS) and Platelet-rich plasma (PRP) on burn stasis zone in an experimental burn model.
Method: Forty rats were divided randomly into four groups. Burns were created in each group according to the comb burn model. The control group received no treatment; the mesenchymal stem cell [MSC] group received MSC; the platelet-rich plasma [PRP] group received PRP; and the ovarian follicular fluid [OFF] group received OFF subcutaneously on days 1, 3 and 5. On days 1 and 21, all rats were photographed and the burn sites were calculated. At the end of day 21, all rats were sacrificed, the dorsum containing the created burns were excised, and the epithelialization, collagen amount, fibroblast density, inflammatory cell density and VEGF amounts were evaluated histopathologically.
Results: The groups were assessed based on the burn site healing rate and histopathological scoring. Healing was faster in the MSC group [p<0.005], PRP group [p>0.005] and OFS group [p<0.005] than in the control group. When the treatment groups were compared with each other, the best healing was observed in the MSC, PRP and OFF groups, respectively.
Conclusions: MSC, PRP and OFF were found to have a positive effect on burn healing, with MSC being the most efficient method among the three, followed by PRP and OFF, respectively, which were found to provide a faster healing than the control group.

Anahtar Kelimeler

Burn Mesenchymal stem cells Platelet-rich plasma Ovarian follicule fluid

Destekleyen Kurum

CUBAP

Proje Numarası

T-758

Kaynakça

  • Refarans1 Pruitt, B. A., Wolf, S. E., & Mason, A. D. [2012]. Epidemiological, demographic, and outcome characteristics of burn injury. Total burn care, 4, 15-45.
  • Refarans2. Jackson, D. M. [1953]. The diagnosis of the depth of burning. British journal of surgery, 40[164], 588-596.
  • Refarans3. Shupp, J. W., Nasabzadeh, T. J., Rosenthal, D. S., Jordan, M. H., Fidler, P., & Jeng, J. C. [2010]. A review of the local pathophysiologic bases of burn wound progression. Journal of burn care & research, 31[6], 849-873.
  • Refarans4. Tan, J. Q., Zhang, H. H., Lei, Z. J., Ren, P., Deng, C., Li, X. Y., & Chen, S. Z. [2013]. The roles of autophagy and apoptosis in burn wound progression in rats. Burns, 39[8], 1551-1556.
  • Refarans5. Zawacki, B. E. [1974]. Reversal of capillary stasis and prevention of necrosis in burns. Annals of surgery, 180[1], 98.
  • Refarans6. Rangatchew, F., Vester-Glowinski, P., Rasmussen, B. S., Haastrup, E., Munthe-Fog, L., Talman, M. L., at all.. (2020). Mesenchymal stem cell therapy of acute thermal burns: A systematic review of the effect on inflammation and wound healing. Burns.Lacci, K. M., & Dardik, A. [2010]. Platelet-rich plasma: support for its use in wound healing. The Yale journal of biology and medicine, 83[1], 1.
  • Refarans7. Hammadeh, M. E., Ertan, A. K., Zeppezauer, M., Baltes, S., Georg, T., Rosenbaum, P., Schmidt, W. [2002]. Immunoglobulins and cytokines level in follicular fluid in relation to etiology of infertility and their relevance to IVF outcome. American Journal of Reproductive Immunology, 47[2], 82-90.
  • Refarans8. Ozornek, M. H., Bielfeld, P., Krüssel, J. S., Hirchenhain, J., Jeyendran, R. S., Koldovsky, U. [1999]. Epidermal growth factor and leukemia inhibitory factor levels in follicular fluid. Association with in vitro fertilization outcome. The Journal of reproductive medicine, 44[4], 367-369.
  • Refarans9. Mendoza, C., Ruiz-Requena, E., Ortega, E., Cremades, N., Martinez, F., Bernabeu, R., et al. [2002]. Follicular fluid markers of oocyte developmental potential. Human Reproduction, 17[4], 1017-1022.
  • Refarans10. Vujisic, S., Zidovec, S. [2005]. Follicular immunology environment and the influence on in vitro fertilization outcome. Current Women's Health Reviews, 1[1], 49-60.
  • Referans11. Calogero, A. E., Nicoletti, F., Palumbo, M. A., Burrello, N., Di Mauro, M., Lunetta, M., et al. [1998]. Macrophage-derived cytokines in the follikuler fluids of women infertility due to immunological causes. Elevated levels of interleukin 6 and low levels of granulocyte-macrophage colony-stimulating factor. Cytokine, 10[10], 814-818.
  • Referans12. Chae, H., Hong, S. H., Hong, S. H., Kim, S. H., Kim, C. H., Kang, B. M., et al. [2007]. Influence of tumor necrosis factor-α on estradiol, progesterone, insulin-like growth factor-II, and insulin-like growth factor binding protein-1, 2, and 3 in cultured human luteinized granulosa cells. European Journal of Obstetrics & Gynecology and Reproductive Biology, 131[2], 176-181.
  • Referans13. Pellicer, A., Albert, C., Mercader, A., Bonilla-Musoles, F., Remohı́, J., Simón, C. [1998]. The follicular and endocrine environment in women with endometriosis: local and systemic cytokine production. Fertility and sterility, 70[3], 425-431.
  • Referans14. Regas, F. C., Ehrlich, H. P. [1992]. Elucidating the vascular response to burns with a new rat model. The Journal of trauma, 32[5], 557-563.
  • Referans15. Pellicer, A., Albert, C., Mercader, A., Bonilla-Musoles, F., Remohí, J., Simón, C. [1999]. The pathogenesis of ovarian hyperstimulation syndrome: in vivo studies investigating the role of interleukin-1β, interleukin-6, and vascular endothelial growth factor. Fertility and sterility, 71[3], 482-489.
  • Referans16. Sharma, V. P., O'Boyle, C. P., Jeffery, S. L. [2011]. Man or machine? The clinimetric properties of laser Doppler imaging in burn depth assessment. Journal of Burn Care & Research, 32[1], 143-149.
  • Referans17. Sparkes, B. G. [1997]. Immunological responses to thermal injury. Burns, 23[2], 106-113.
  • Referans18. Nagato, H., Umebayashi, Y., Wako, M., Tabata, Y., Manabe, M. [2006]. Collagen–poly glycolic acid hybrid matrix with basic fibroblast growth factor accelerated angiogenesis and granulation tissue formation in diabetic mice. The Journal of dermatology, 33[10], 670-675.
  • Referans19. Nursal T.Z., Baykal A., Hamaloğlu E. Wound Healing in the Elderly: Is there a difference? Turkish Journal of Geriatrics Geriatri, 2[1]: 2932, [1999].
  • Referans20. Rodgers, K., Jadhav, S. S. [2018]. The application of mesenchymal stem cells to treat thermal and radiation burns. Advanced drug delivery reviews, 123, 75-81.
  • Referans21. Verstappen, J., Katsaros, C., Torensma, R., Von den Hoff, J. W. [2009]. A functional model for adult stem cells in epithelial tissues. Wound repair and regeneration, 17[3], 296-305.
  • Referans22. Wu, Y., Chen, L., Scott, P. G., Tredget, E. E. [2007]. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem cells, 25[10], 2648-2659.
  • Referans23. . Zuk, P. A., Zhu, M. I. N., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., et al. [2001]. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue engineering, 7[2], 211-228..
  • Referans24. Shumakov, V. I., Onishchenko, N. A., Rasulov, M. F., Krasheninnikov, M. E., & Zaidenov, V. A. [2003]. Mesenchymal bone marrow stem cells more effectively stimulate regeneration of deep burn wounds than embryonic fibroblasts. Bulletin of experimental biology and medicine, 136[2], 192-195.
  • Referans25. Falanga, V., Iwamoto, S., Chartier, M., Yufit, T., Butmarc, J., Kouttab, N., et al. [2007]. Autologous bone marrow–derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue engineering, 13[6], 1299-1312.
  • Referans26. Lu, D., Chen, B., Liang, Z., Deng, W., Jiang, Y., Li, S., et al. [2011]. Comparison of bone marrow mesenchymal stem cells with bone marrow-derived mononuclear cells for treatment of diabetic critical limb ischemia and foot ulcer: a double-blind, randomized, controlled trial. Diabetes research and clinical practice, 92[1], 26-36.
  • Referans27. Volarevic, V., Arsenijevic, N., Lukic, M. L., Stojkovic, M. [2011]. Concise review: mesenchymal stem cell treatment of the complications of diabetes mellitus. Stem cells, 29[1], 5-10.
  • Referans28. Lataillade, J. J., Doucet, C., Bey, E., Carsin, H., Huet, C., Clairand, I., et al. [2007]. New approach to radiation burn treatment by dosimetry-guided surgery combined with autologous mesenchymal stem cell therapy.
  • Referans29. Vojtaššák, J., Danišovič, L., Kubeš, M., Bakoš, D., Jarabek, L., Uličná, M., et al. [2006]. Autologous biograft and mesenchymal stem cells in treatment of the diabetic foot. Neuroendocrinology Letters, 27[supplement 2], 134-137.
  • Referans30. Nurden, A. T., Nurden, P., Sanchez, M., Andia, I., Anitua, E. [2008]. Platelets and wound healing. Frontiers in bioscience: a journal and virtual library, 13, 3532-3548.
  • Referans31. Peavy, G. M., Jacobson, M. W., Salmon, D. P., Gamst, A. C., Patterson, T. L., Goldman, S., et al. [2012]. The influence of chronic stress on dementia-related diagnostic change in older adults. Alzheimer disease and associated disorders, 26[3], 260..
  • Referans32. Su, C. Y., Kuo, Y. P., Nieh, H. L., Tseng, Y. H., Burnouf, T. [2008]. Quantitative assessment of the kinetics of growth factors release from platelet gel. Transfusion, 48[11], 2414-2420.
  • Referans33. Zimmermann, R., Arnold, D., Strasser, E., Ringwald, J., Schlegel, A., Wiltfang, J., et al. [2003]. Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates. Vox sanguinis, 85[4], 283-289.
  • Referans34. Tschon, M., Fini, M., Giardino, R., Filardo, G., Dallari, D., Torricelli, P., et al. [2011]. [Frontiers in Bioscience E3, 96-107, January 1, 2011] Lights and shadows concerning platelet products for musculoskeletal regeneration. Frontiers in Bioscience, 3, 96-107.
  • Referans35. Mazzucco, L., Borzini, P., Gope, R. [2010]. Platelet-derived factors involved in tissue repair—from signal to function. Transfusion medicine reviews, 24[3], 218-234. 105[S 06], S13-S33.
  • Referans36. Nurden, A. T. [2011]. Platelets, inflammation and tissue regeneration. Thrombosis and haemostasis, 105[S 06], S13-S33.
  • Referans37. Henderson, J. L., Cupp, C. L., Ross, E. V., Shick, P. C., Keefe, M. A., Wester, D. C., et al. [2003]. The effects of autologous platelet gel on wound healing. Ear, nose & throat journal, 82[8], 598-602.
  • Referans38. Kazakos, K., Lyras, D. N., Verettas, D., Tilkeridis, K., & Tryfonidis, M. [2009]. The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury, 40[8], 801-805.
  • Referans39. Liu, J., Qu, W., Li, R., Zheng, C., Zhang, L. [2018]. Efficacy of autologous platelet-rich gel in the treatment of deep grade II burn wounds. Int J Clin Exp Med, 11[3], 2654-2659.
  • Referans40. Singer, A. J., Toussaint, J., Chung, W. T., McClain, S., Raut, V., Rosenberg, L. [2018]. The effects of platelet rich plasma on healing of full thickness burns in swine. Burns, 44[6], 1543-1550.
  • Referans41. Marck, R. E., Middelkoop, E., & Breederveld, R. S. [2014]. Considerations on the use of platelet-rich plasma, specifically for burn treatment. Journal of Burn Care & Research, 35[3], 219-227.
  • Referans42. Moncayo HE, Penz-Koza A, Marth C, Gastl G, Herold M,Moncayo R. Vascular endothelial growth factor in serum and in the follicular fluid of patients undergoing hormonal stimulation for invitro fertilization: Hum Reprod 1998; 13[12]: 3310-4.
  • Referans43. Ayhan S. [2007]. Primer yara iyileşmesi üzerine embriyonik kök hücre ve ovaryum folikül sıvısının etkisi, Yayınlanmamış Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi, Eskişehir.

Yıl 2021, Cilt: 43 Sayı: 1, 62 - 71, 31.03.2021

Hüsnü Genç Sinan Soylu , Deniz Şahin İnan , Atilla Kurt , Hakkı Çoşkun , Ali Yıldırır

https://doi.org/10.7197/cmj.904376

Öz

Proje Numarası

T-758

Kaynakça

  • Refarans1 Pruitt, B. A., Wolf, S. E., & Mason, A. D. [2012]. Epidemiological, demographic, and outcome characteristics of burn injury. Total burn care, 4, 15-45.
  • Refarans2. Jackson, D. M. [1953]. The diagnosis of the depth of burning. British journal of surgery, 40[164], 588-596.
  • Refarans3. Shupp, J. W., Nasabzadeh, T. J., Rosenthal, D. S., Jordan, M. H., Fidler, P., & Jeng, J. C. [2010]. A review of the local pathophysiologic bases of burn wound progression. Journal of burn care & research, 31[6], 849-873.
  • Refarans4. Tan, J. Q., Zhang, H. H., Lei, Z. J., Ren, P., Deng, C., Li, X. Y., & Chen, S. Z. [2013]. The roles of autophagy and apoptosis in burn wound progression in rats. Burns, 39[8], 1551-1556.
  • Refarans5. Zawacki, B. E. [1974]. Reversal of capillary stasis and prevention of necrosis in burns. Annals of surgery, 180[1], 98.
  • Refarans6. Rangatchew, F., Vester-Glowinski, P., Rasmussen, B. S., Haastrup, E., Munthe-Fog, L., Talman, M. L., at all.. (2020). Mesenchymal stem cell therapy of acute thermal burns: A systematic review of the effect on inflammation and wound healing. Burns.Lacci, K. M., & Dardik, A. [2010]. Platelet-rich plasma: support for its use in wound healing. The Yale journal of biology and medicine, 83[1], 1.
  • Refarans7. Hammadeh, M. E., Ertan, A. K., Zeppezauer, M., Baltes, S., Georg, T., Rosenbaum, P., Schmidt, W. [2002]. Immunoglobulins and cytokines level in follicular fluid in relation to etiology of infertility and their relevance to IVF outcome. American Journal of Reproductive Immunology, 47[2], 82-90.
  • Refarans8. Ozornek, M. H., Bielfeld, P., Krüssel, J. S., Hirchenhain, J., Jeyendran, R. S., Koldovsky, U. [1999]. Epidermal growth factor and leukemia inhibitory factor levels in follicular fluid. Association with in vitro fertilization outcome. The Journal of reproductive medicine, 44[4], 367-369.
  • Refarans9. Mendoza, C., Ruiz-Requena, E., Ortega, E., Cremades, N., Martinez, F., Bernabeu, R., et al. [2002]. Follicular fluid markers of oocyte developmental potential. Human Reproduction, 17[4], 1017-1022.
  • Refarans10. Vujisic, S., Zidovec, S. [2005]. Follicular immunology environment and the influence on in vitro fertilization outcome. Current Women's Health Reviews, 1[1], 49-60.
  • Referans11. Calogero, A. E., Nicoletti, F., Palumbo, M. A., Burrello, N., Di Mauro, M., Lunetta, M., et al. [1998]. Macrophage-derived cytokines in the follikuler fluids of women infertility due to immunological causes. Elevated levels of interleukin 6 and low levels of granulocyte-macrophage colony-stimulating factor. Cytokine, 10[10], 814-818.
  • Referans12. Chae, H., Hong, S. H., Hong, S. H., Kim, S. H., Kim, C. H., Kang, B. M., et al. [2007]. Influence of tumor necrosis factor-α on estradiol, progesterone, insulin-like growth factor-II, and insulin-like growth factor binding protein-1, 2, and 3 in cultured human luteinized granulosa cells. European Journal of Obstetrics & Gynecology and Reproductive Biology, 131[2], 176-181.
  • Referans13. Pellicer, A., Albert, C., Mercader, A., Bonilla-Musoles, F., Remohı́, J., Simón, C. [1998]. The follicular and endocrine environment in women with endometriosis: local and systemic cytokine production. Fertility and sterility, 70[3], 425-431.
  • Referans14. Regas, F. C., Ehrlich, H. P. [1992]. Elucidating the vascular response to burns with a new rat model. The Journal of trauma, 32[5], 557-563.
  • Referans15. Pellicer, A., Albert, C., Mercader, A., Bonilla-Musoles, F., Remohí, J., Simón, C. [1999]. The pathogenesis of ovarian hyperstimulation syndrome: in vivo studies investigating the role of interleukin-1β, interleukin-6, and vascular endothelial growth factor. Fertility and sterility, 71[3], 482-489.
  • Referans16. Sharma, V. P., O'Boyle, C. P., Jeffery, S. L. [2011]. Man or machine? The clinimetric properties of laser Doppler imaging in burn depth assessment. Journal of Burn Care & Research, 32[1], 143-149.
  • Referans17. Sparkes, B. G. [1997]. Immunological responses to thermal injury. Burns, 23[2], 106-113.
  • Referans18. Nagato, H., Umebayashi, Y., Wako, M., Tabata, Y., Manabe, M. [2006]. Collagen–poly glycolic acid hybrid matrix with basic fibroblast growth factor accelerated angiogenesis and granulation tissue formation in diabetic mice. The Journal of dermatology, 33[10], 670-675.
  • Referans19. Nursal T.Z., Baykal A., Hamaloğlu E. Wound Healing in the Elderly: Is there a difference? Turkish Journal of Geriatrics Geriatri, 2[1]: 2932, [1999].
  • Referans20. Rodgers, K., Jadhav, S. S. [2018]. The application of mesenchymal stem cells to treat thermal and radiation burns. Advanced drug delivery reviews, 123, 75-81.
  • Referans21. Verstappen, J., Katsaros, C., Torensma, R., Von den Hoff, J. W. [2009]. A functional model for adult stem cells in epithelial tissues. Wound repair and regeneration, 17[3], 296-305.
  • Referans22. Wu, Y., Chen, L., Scott, P. G., Tredget, E. E. [2007]. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem cells, 25[10], 2648-2659.
  • Referans23. . Zuk, P. A., Zhu, M. I. N., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., et al. [2001]. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue engineering, 7[2], 211-228..
  • Referans24. Shumakov, V. I., Onishchenko, N. A., Rasulov, M. F., Krasheninnikov, M. E., & Zaidenov, V. A. [2003]. Mesenchymal bone marrow stem cells more effectively stimulate regeneration of deep burn wounds than embryonic fibroblasts. Bulletin of experimental biology and medicine, 136[2], 192-195.
  • Referans25. Falanga, V., Iwamoto, S., Chartier, M., Yufit, T., Butmarc, J., Kouttab, N., et al. [2007]. Autologous bone marrow–derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue engineering, 13[6], 1299-1312.
  • Referans26. Lu, D., Chen, B., Liang, Z., Deng, W., Jiang, Y., Li, S., et al. [2011]. Comparison of bone marrow mesenchymal stem cells with bone marrow-derived mononuclear cells for treatment of diabetic critical limb ischemia and foot ulcer: a double-blind, randomized, controlled trial. Diabetes research and clinical practice, 92[1], 26-36.
  • Referans27. Volarevic, V., Arsenijevic, N., Lukic, M. L., Stojkovic, M. [2011]. Concise review: mesenchymal stem cell treatment of the complications of diabetes mellitus. Stem cells, 29[1], 5-10.
  • Referans28. Lataillade, J. J., Doucet, C., Bey, E., Carsin, H., Huet, C., Clairand, I., et al. [2007]. New approach to radiation burn treatment by dosimetry-guided surgery combined with autologous mesenchymal stem cell therapy.
  • Referans29. Vojtaššák, J., Danišovič, L., Kubeš, M., Bakoš, D., Jarabek, L., Uličná, M., et al. [2006]. Autologous biograft and mesenchymal stem cells in treatment of the diabetic foot. Neuroendocrinology Letters, 27[supplement 2], 134-137.
  • Referans30. Nurden, A. T., Nurden, P., Sanchez, M., Andia, I., Anitua, E. [2008]. Platelets and wound healing. Frontiers in bioscience: a journal and virtual library, 13, 3532-3548.
  • Referans31. Peavy, G. M., Jacobson, M. W., Salmon, D. P., Gamst, A. C., Patterson, T. L., Goldman, S., et al. [2012]. The influence of chronic stress on dementia-related diagnostic change in older adults. Alzheimer disease and associated disorders, 26[3], 260..
  • Referans32. Su, C. Y., Kuo, Y. P., Nieh, H. L., Tseng, Y. H., Burnouf, T. [2008]. Quantitative assessment of the kinetics of growth factors release from platelet gel. Transfusion, 48[11], 2414-2420.
  • Referans33. Zimmermann, R., Arnold, D., Strasser, E., Ringwald, J., Schlegel, A., Wiltfang, J., et al. [2003]. Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates. Vox sanguinis, 85[4], 283-289.
  • Referans34. Tschon, M., Fini, M., Giardino, R., Filardo, G., Dallari, D., Torricelli, P., et al. [2011]. [Frontiers in Bioscience E3, 96-107, January 1, 2011] Lights and shadows concerning platelet products for musculoskeletal regeneration. Frontiers in Bioscience, 3, 96-107.
  • Referans35. Mazzucco, L., Borzini, P., Gope, R. [2010]. Platelet-derived factors involved in tissue repair—from signal to function. Transfusion medicine reviews, 24[3], 218-234. 105[S 06], S13-S33.
  • Referans36. Nurden, A. T. [2011]. Platelets, inflammation and tissue regeneration. Thrombosis and haemostasis, 105[S 06], S13-S33.
  • Referans37. Henderson, J. L., Cupp, C. L., Ross, E. V., Shick, P. C., Keefe, M. A., Wester, D. C., et al. [2003]. The effects of autologous platelet gel on wound healing. Ear, nose & throat journal, 82[8], 598-602.
  • Referans38. Kazakos, K., Lyras, D. N., Verettas, D., Tilkeridis, K., & Tryfonidis, M. [2009]. The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury, 40[8], 801-805.
  • Referans39. Liu, J., Qu, W., Li, R., Zheng, C., Zhang, L. [2018]. Efficacy of autologous platelet-rich gel in the treatment of deep grade II burn wounds. Int J Clin Exp Med, 11[3], 2654-2659.
  • Referans40. Singer, A. J., Toussaint, J., Chung, W. T., McClain, S., Raut, V., Rosenberg, L. [2018]. The effects of platelet rich plasma on healing of full thickness burns in swine. Burns, 44[6], 1543-1550.
  • Referans41. Marck, R. E., Middelkoop, E., & Breederveld, R. S. [2014]. Considerations on the use of platelet-rich plasma, specifically for burn treatment. Journal of Burn Care & Research, 35[3], 219-227.
  • Referans42. Moncayo HE, Penz-Koza A, Marth C, Gastl G, Herold M,Moncayo R. Vascular endothelial growth factor in serum and in the follicular fluid of patients undergoing hormonal stimulation for invitro fertilization: Hum Reprod 1998; 13[12]: 3310-4.
  • Referans43. Ayhan S. [2007]. Primer yara iyileşmesi üzerine embriyonik kök hücre ve ovaryum folikül sıvısının etkisi, Yayınlanmamış Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi, Eskişehir.
Toplam 43 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Surgical Science Research Makaleler
Yazarlar

Hüsnü Genç 0000-0001-9514-7096

Sinan Soylu 0000-0002-3911-3227

Deniz Şahin İnan 0000-0002-0292-4448

Atilla Kurt 0000-0002-3649-6293

Hakkı Çoşkun 0000-0001-7913-8325

Ali Yıldırır 0000-0001-8228-2862

Proje Numarası T-758
Yayımlanma Tarihi 31 Mart 2021
Kabul Tarihi 31 Mart 2021
Yayımlandığı Sayı Yıl 2021Cilt: 43 Sayı: 1

Kaynak Göster

AMA Genç H, Soylu S, Şahin İnan D, Kurt A, Çoşkun H, Yıldırır A. Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study). CMJ. Mart 2021;43(1):62-71. doi:10.7197/cmj.904376