Redirecting to http://dergipark.org.tr/_fragment?_hash=5b5MAJlhDCWpBQ_ZM-YF_O31QS7mOLl8sBUJFfsoM_A&_path=id%3D347461%26_format%3Dhtml%26_locale%3Den%26_controller%3DApp%255CController%255CSite%255CArticleController%253A%253AesiArticleStats.
Research Article
BibTex RIS Cite

Ratlarda deneysel periodontitis modellerinde sistemik ve lokal interlökin 1BETA(IL-1β) ve IL-10 seviyelerine ozonun etkisi

Year 2017, Volume: 39 Issue: 3, 608 - 619, 19.09.2017

Aysun Akpınar , Metin Çalışır Ömer Poyraz Fahrettin Göze Derya Özdemir Doğan Vildan Bostancı

https://doi.org/10.7197/223.v39i31705.347461
Cited By: 2

Abstract


Amaç: Bu çalışmanın amacı deneysel periodontitisli ratlarda ozonun
uygulanmasıyla histopatolojik ve morfometrik değişiklikleri analiz etmektir.

Yöntem: 24 rat rastgele olarak üç gruba ayrıldı. Bu gruplar:
ligatürsüz tedavi grubu (NL) (n=8), sadece ligatür uygulanan tedavi grubu (LO)
(n=8) ve ligatür+ozon uygulanan tedavi grubu olarak belirlendi (LOZ) (n=8).
Deneysel periodontitis oluşturmak için ratların sağ mandibular 1. molarlarının
gingival marjinlerine 4/0 ipek sutur yerleştirildi. 14 gün sonra ratlar sakrifiye
edildi. Her gruptaki ratların alveolar kemik seviyelerindeki değişiklikler
klinik olarak ölçüldü, ve her gruptaki ratların osteoklast morfolojileri,
osteoklast ve osteoblast aktiviteleri, enflamatuar hücre infiltrasyonlarını
(ICI) belirlemek için dokuları histopatolojik olarak incelendi. Serum ve
sitokin seviyeleri ratlara özel IL-1β  ve
IL-10 Eliza kitleri kullanılarak ölçüldü.

Bulgular: Mandibular diş çevresindeki alveolar kemik kaybı NL ve
LOZ grupları ile karşılaştırıldığında LO grubunda önemli oranda yüksek bulundu.
(p<0.05) Osteoklast sayıları ve ICI nın mevcudiyetindeki oran NL ve LOZ
gruplarındakinden, LO grubunda önemli oranda yüksek olduğu tespit edildi.
(p<0.05) Osteoblastik aktivite NL ve LOZ gruplarından,  LO grubunda önemli oranda düşük bulundu.  (p<0.05) LO grubundaki serum ve gingival
homojenatdaki IL-1β seviyesi NL ve LOZ grubundakinden önemli oranda yüksek
bulundu. (p<0.05)  LO grubundaki serum
IL-10 seviyesi, NL ve LOZ gruplarındakinden istatistiksel olarak düşüktü.
(p<0.05) NL grubundaki gingival homojenat IL-10 seviyesi LO grubundakinden
istatistiksel olarak yüksek olduğu belirlendi. (p<0.05)  









Sonuç: Mevcut çalışma, ozonun oral uygulamasının rat periodontitis
modellerinde alveolar kemik rezorpsiyonunu azaltığını göstermiştir.

Keywords

Deneysel periodontitis rat ozon IL-1β ve IL-10

References

  • 1. Duang XY, Wang Q, Zhou XD, Huang DM. Mangiferin: a possible strategy for periodontal disease to therapy. Med Hypotheses 2011; 76: 486-88.
  • 2. Breivik T, Gundersen Y, Osmundsen H, Fonnum F, Opstad PK. Neonatal dexamethasone and chronic tianeptine treatment inhibit ligature-induced periodontitis in adult rats. J Periodontal Res 2006; 41: 23-32.
  • 3. Kinane DF, Causation and pathogenesis of periodontal disease. Periodontol 2000 2001; 25: 8-20.
  • 4. Page RC, The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodontal Res 1991; 26: 230-42.
  • 5. Gursoy UK, Könönen E, Uitto V-J. Stimulation of epithelial cell matrix metalloproteinase (MMP-2, -9, -13) and interleukin-8 secretion by fusobacteria. Oral Microbiol Immunol 2008: 23: 432-34.
  • 6. Dewhirst FE, Stashenko PP, Mole JE, Tsurumachi T. Purification and partial sequence of human osteoclast-activating factor: identity with interleukin 1 beta. J Immunol. 1985; 135: 2562-8.
  • 7. Matsuki Y, Yamamoto T, Hara K. Interleukin-1 mRNA-expressing macrophages in human chronically inflamed gingival tissues. Am J Pathol 1991; 138: 1299-305.
  • 8. Matsuki Y, Yamamoto T, Hara K. Detection of inflammatory cytokine messenger RNA (mRNA)-expressing cells in human inflamed gingiva by combined in situ hybridization and immunohistochemistry. Immunology 1992; 76: 42-7.
  • 9. Hönig J, Rordorf-Adam C, Siegmund C, Wiedemann W, Erard F. Increased interleukin-1 beta (IL-1 beta) concentration in gingival tissue from periodontitis patients. J Periodont Res 1989; 24: 362-67.
  • 10. Tokoro Y, Yamamoto T, Hara K. IL-1 beta mRNA as the predominant inflammatory cytokine transcript: correlation with inflammatory cell infiltration into human gingiva. J Oral Pathol Med 1996; 25: 225-31.
  • 11. Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol 2011; 38: 60-84.
  • 12. Arai T, Hiromatsu K, Kobayashi N, Takano M, Ishida H, Nimura Y, Yoshikai Y. IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury. J Immunol 1995; 155: 5743-9.
  • 13. Tumpey TM, Elner VM, Chen SH, Oakes JE, Lausch RN. Interleukin-10 treatment can suppress stromal keratitis induced by herpes simplex virus type 1. J Immunol 1994; 153: 2258-65.
  • 14. Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006; 37: 425-35.
  • 15. Seidler V, Linetskiy I, Hubálková H, Stanková H, Smucler R, Mazánek J. Ozone and its usage in general medicine and dentistry. A review article. Prague Med Rep 2008; 109: 5-13.
  • 16. Adusumilli S, Manthena S, and Anumolu V. Application of ozone in the treatment of periodontal disease J Pharm Bioallied Sci 2013; 5: 89-94.
  • 17. Azarpazhooh A, Limeback H, The application of ozone in dentistry: a systematic review of literature. J Dent 2008; 36: 104-16.
  • 18. Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, Hickel R, Brand K. Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 2006; 114: 435-40.
  • 19. Sagai M, Bocci V. Mechanisms of Action Involved in Ozone Therapy: Is healing induced via a mild oxidative stress? Med Gas Res 2011; 20: 1-29.
  • 20. Polydorou O, Halili A, Wittmer A, Pelz K, Hahn P. The antibacterial effect of gas ozone after 2 months of in vitro evaluation. Clin Oral Investig 2012; 16: 545-50.
  • 21. Polydorou O, Pelz K, Hahn P. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci 2006; 114: 349-53.
  • 22. Baysan A, Lynch E. Effect of ozone on the oral microbiota and clinical severity of primary root caries. Am J Dent 2004; 17: 56-60.
  • 23. Sakallıoğlu E.E, Ayas B, Lütfioğlu M, Keleş G.Ç, Açıkgöz G, Fıratlı E. Gingival levels of monocyte chemoattractant protein-1 (MCP-1) in diabetes mellitus and periodontitis: an experimental study in rats. Clin Oral Invest 2008; 12: 83-89.
  • 24. de Lima V, Bezerra MM, de Menezes Alencar VB, Vidal FD, da Rocha FA, de Castro Brito GA, de Albuquerque Ribeiro R. Effects of chlorpromazine on alveolar bone loss in experimental periodontal disease in rats. Eur J Oral Sci 2000; 108: 123-9.
  • 25. Buduneli E, Vardar S, Buduneli N, Berdeli AH, Türkoğlu O, Başkesen A, Atilla G. Effects of combined systemic administration of low-dose doxycycline and alendronate on endotoxin-induced periodontitis in rats. J Periodontol 2004; 75: 1516-23.
  • 26. Holzhausen M, Spolidorio DMP, Muscara MN, Hebling J, Spolidorio LC. Protective effects of etoricoxib, a selective inhibitor of cyclooxygenase-2, in experimental periodontitis in rats. J Periodontal Res 2005; 40: 208-11.
  • 27. Xu Y, Wei W. A comparative study of systemic subantimicrobial and topical treatment of minocycline in experimental periodontitis of rats. Arch Oral Biol 2006; 51: 794-803.
  • 28. Di Paola R, Mazzon E, Zito D, Maiere D, Britti D, Genovese T, Cuzzocrea S. Effects of Tempol, a membrane-permeable radical scavenger, in a rodent model periodontitis. J Clin Periodontol 2005; 32: 1062-68.
  • 29. Menabde GT, Natroshvili ND, Natroshvili TD. [Ozonotherapy for the treatment of parodontitis]. Georgian Med News 2006; 134: 43-6.
  • 30.Brauner AW. Periodontology: New Methods. Ozone: Science &Engineering 1992; 14: 165-76.
  • 31. Pahl HL. Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene. 1999; 18: 6853-66.
  • 32. Gamonal J, Acevedo A, Bascones A, Jorge O, Silva A. Levels of interleukin-1 beta, -8, and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J Periodontol 2000; 71: 1535-45.
  • 33. Okada H, Murakami S. Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med 1998; 9: 248-66.
  • 34. Bartold PM, Narayanan AS. Molecular and cell biology of healthy and diseased periodontal tissues. Periodontol 2000. 2006; 40: 29-49.
  • 35. Graves DT, Jiang Y, Genco C. Periodontal disease: bacterial virulence factors, host response and impact on systemic health. Curr Opin Infect Dis 2000; 13: 227-32.
  • 36. Millar BJ, Hodson N. Assessment of the safety of two ozone delivery devices. J Dent 2007; 35: 195-200.
  • 37. Sharma M, Hudson JB. Ozone gas is an effective and practical antibacterial agent. Am J Infect Control 2008; 36: 559-63.
  • 38. Baysan A, Lynch E. The use of ozone in dentistry and medicine. Prim Dent Care 2005; 12: 47-52.
  • 39. Müller P, Guggenheim B, Schmidlin PR. Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. Eur J Oral Sci 2007; 115: 77-80.
  • 40. Matsumura K, Hyon SH, Nakajima N, Iwata H, Watazu A, Tsutsumi S. Surface modification of poly(ethylene-co-vinyl alcohol): hydroxyapatite immobilization and control of periodontal ligament cells differentiation. Biomaterials. 2004; 25: 4817-24.
  • 41. Matsumura K, Hyon SH, Nakajima N, Tsutsumi S. Effects on gingival cells of hydroxyapatite immobilized on poly(ethylene-co-vinyl alcohol). J Biomed Mater Res A 2007; 82: 288-95.
  • 42. Fillipi A. The influence of ozonised water on the epithelial wound healing process in the oral cavity. Radiology and Oral Medicine, 2001.

The effects of ozone on the local and systemic interleukin 1β (IL-1β) and IL-10 levels experimental periodontitis model in rats

Year 2017, Volume: 39 Issue: 3, 608 - 619, 19.09.2017

Aysun Akpınar , Metin Çalışır Ömer Poyraz Fahrettin Göze Derya Özdemir Doğan Vildan Bostancı

https://doi.org/10.7197/223.v39i31705.347461
Cited By: 2

Abstract

 


Aim: The purpose of this study was to analyze histologically the effect of ozone therapy on alveolar bone loss and İnterleukin 1β (IL-1β), IL-10 in experimental periodontitis in a rat model.

Material and Methods: Twenty-four rats were randomly divided into three experimental groups: a nonligated (NL) treatment group (n = 8), a ligature-only (LO) treatment group (n = 8), and a ligature plus ozone (60 second/each tooth a day) (LOZ) treatment group. In order to induce experimental periodontitis, a 4/0 silk suture was placed at the gingival margin of the right-mandibular first molars of the rats. The study duration was 14 days, and then the animals were sacrified. Changes in the alveolar bone levels of rats in each group were measured clinically, and the tissues of the rats in each group were examined histopathologically to determine inflammatory cell infiltration (ICI), osteoblast and osteoclast activities, and osteoclast morphology. Serum and gingival cytokine levels were measured by using the rat-specific IL-1β and IL-10 ELISA kits.

Results: Alveolar bone loss around the mandibular molar tooth was significantly higher in the LO group compared with NL and LOZ groups (p<0.05). The ratio of the presence of ICI and osteoclast numbers were significantly higher in the LO group than in the NL and LOZ groups (p<0.05). Osteoblastic activity was significantly lower in the LO group than in the NL and LOZ groups (p<0.05). The serum and gingival homogenate IL-1β levels in the LO group were statistically higher than in the NL and LOZ groups (p<0.05). The serum IL-10 level in the LO group was statistically lower than in the NL and LOZ groups (p<0.05). The gingival homogenate IL-10 level in NL group was statistically higher than in the LO group (p<0.05).

Conclusion: The present study showed that the oral administration of ozone diminishes alveolar bone resorption in a rat periodontitis model.

 

Keywords

Experimental periodontitis rat ozone IL-1β IL-10

References

  • 1. Duang XY, Wang Q, Zhou XD, Huang DM. Mangiferin: a possible strategy for periodontal disease to therapy. Med Hypotheses 2011; 76: 486-88.
  • 2. Breivik T, Gundersen Y, Osmundsen H, Fonnum F, Opstad PK. Neonatal dexamethasone and chronic tianeptine treatment inhibit ligature-induced periodontitis in adult rats. J Periodontal Res 2006; 41: 23-32.
  • 3. Kinane DF, Causation and pathogenesis of periodontal disease. Periodontol 2000 2001; 25: 8-20.
  • 4. Page RC, The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodontal Res 1991; 26: 230-42.
  • 5. Gursoy UK, Könönen E, Uitto V-J. Stimulation of epithelial cell matrix metalloproteinase (MMP-2, -9, -13) and interleukin-8 secretion by fusobacteria. Oral Microbiol Immunol 2008: 23: 432-34.
  • 6. Dewhirst FE, Stashenko PP, Mole JE, Tsurumachi T. Purification and partial sequence of human osteoclast-activating factor: identity with interleukin 1 beta. J Immunol. 1985; 135: 2562-8.
  • 7. Matsuki Y, Yamamoto T, Hara K. Interleukin-1 mRNA-expressing macrophages in human chronically inflamed gingival tissues. Am J Pathol 1991; 138: 1299-305.
  • 8. Matsuki Y, Yamamoto T, Hara K. Detection of inflammatory cytokine messenger RNA (mRNA)-expressing cells in human inflamed gingiva by combined in situ hybridization and immunohistochemistry. Immunology 1992; 76: 42-7.
  • 9. Hönig J, Rordorf-Adam C, Siegmund C, Wiedemann W, Erard F. Increased interleukin-1 beta (IL-1 beta) concentration in gingival tissue from periodontitis patients. J Periodont Res 1989; 24: 362-67.
  • 10. Tokoro Y, Yamamoto T, Hara K. IL-1 beta mRNA as the predominant inflammatory cytokine transcript: correlation with inflammatory cell infiltration into human gingiva. J Oral Pathol Med 1996; 25: 225-31.
  • 11. Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol 2011; 38: 60-84.
  • 12. Arai T, Hiromatsu K, Kobayashi N, Takano M, Ishida H, Nimura Y, Yoshikai Y. IL-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury. J Immunol 1995; 155: 5743-9.
  • 13. Tumpey TM, Elner VM, Chen SH, Oakes JE, Lausch RN. Interleukin-10 treatment can suppress stromal keratitis induced by herpes simplex virus type 1. J Immunol 1994; 153: 2258-65.
  • 14. Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006; 37: 425-35.
  • 15. Seidler V, Linetskiy I, Hubálková H, Stanková H, Smucler R, Mazánek J. Ozone and its usage in general medicine and dentistry. A review article. Prague Med Rep 2008; 109: 5-13.
  • 16. Adusumilli S, Manthena S, and Anumolu V. Application of ozone in the treatment of periodontal disease J Pharm Bioallied Sci 2013; 5: 89-94.
  • 17. Azarpazhooh A, Limeback H, The application of ozone in dentistry: a systematic review of literature. J Dent 2008; 36: 104-16.
  • 18. Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, Hickel R, Brand K. Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 2006; 114: 435-40.
  • 19. Sagai M, Bocci V. Mechanisms of Action Involved in Ozone Therapy: Is healing induced via a mild oxidative stress? Med Gas Res 2011; 20: 1-29.
  • 20. Polydorou O, Halili A, Wittmer A, Pelz K, Hahn P. The antibacterial effect of gas ozone after 2 months of in vitro evaluation. Clin Oral Investig 2012; 16: 545-50.
  • 21. Polydorou O, Pelz K, Hahn P. Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci 2006; 114: 349-53.
  • 22. Baysan A, Lynch E. Effect of ozone on the oral microbiota and clinical severity of primary root caries. Am J Dent 2004; 17: 56-60.
  • 23. Sakallıoğlu E.E, Ayas B, Lütfioğlu M, Keleş G.Ç, Açıkgöz G, Fıratlı E. Gingival levels of monocyte chemoattractant protein-1 (MCP-1) in diabetes mellitus and periodontitis: an experimental study in rats. Clin Oral Invest 2008; 12: 83-89.
  • 24. de Lima V, Bezerra MM, de Menezes Alencar VB, Vidal FD, da Rocha FA, de Castro Brito GA, de Albuquerque Ribeiro R. Effects of chlorpromazine on alveolar bone loss in experimental periodontal disease in rats. Eur J Oral Sci 2000; 108: 123-9.
  • 25. Buduneli E, Vardar S, Buduneli N, Berdeli AH, Türkoğlu O, Başkesen A, Atilla G. Effects of combined systemic administration of low-dose doxycycline and alendronate on endotoxin-induced periodontitis in rats. J Periodontol 2004; 75: 1516-23.
  • 26. Holzhausen M, Spolidorio DMP, Muscara MN, Hebling J, Spolidorio LC. Protective effects of etoricoxib, a selective inhibitor of cyclooxygenase-2, in experimental periodontitis in rats. J Periodontal Res 2005; 40: 208-11.
  • 27. Xu Y, Wei W. A comparative study of systemic subantimicrobial and topical treatment of minocycline in experimental periodontitis of rats. Arch Oral Biol 2006; 51: 794-803.
  • 28. Di Paola R, Mazzon E, Zito D, Maiere D, Britti D, Genovese T, Cuzzocrea S. Effects of Tempol, a membrane-permeable radical scavenger, in a rodent model periodontitis. J Clin Periodontol 2005; 32: 1062-68.
  • 29. Menabde GT, Natroshvili ND, Natroshvili TD. [Ozonotherapy for the treatment of parodontitis]. Georgian Med News 2006; 134: 43-6.
  • 30.Brauner AW. Periodontology: New Methods. Ozone: Science &Engineering 1992; 14: 165-76.
  • 31. Pahl HL. Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene. 1999; 18: 6853-66.
  • 32. Gamonal J, Acevedo A, Bascones A, Jorge O, Silva A. Levels of interleukin-1 beta, -8, and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J Periodontol 2000; 71: 1535-45.
  • 33. Okada H, Murakami S. Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med 1998; 9: 248-66.
  • 34. Bartold PM, Narayanan AS. Molecular and cell biology of healthy and diseased periodontal tissues. Periodontol 2000. 2006; 40: 29-49.
  • 35. Graves DT, Jiang Y, Genco C. Periodontal disease: bacterial virulence factors, host response and impact on systemic health. Curr Opin Infect Dis 2000; 13: 227-32.
  • 36. Millar BJ, Hodson N. Assessment of the safety of two ozone delivery devices. J Dent 2007; 35: 195-200.
  • 37. Sharma M, Hudson JB. Ozone gas is an effective and practical antibacterial agent. Am J Infect Control 2008; 36: 559-63.
  • 38. Baysan A, Lynch E. The use of ozone in dentistry and medicine. Prim Dent Care 2005; 12: 47-52.
  • 39. Müller P, Guggenheim B, Schmidlin PR. Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. Eur J Oral Sci 2007; 115: 77-80.
  • 40. Matsumura K, Hyon SH, Nakajima N, Iwata H, Watazu A, Tsutsumi S. Surface modification of poly(ethylene-co-vinyl alcohol): hydroxyapatite immobilization and control of periodontal ligament cells differentiation. Biomaterials. 2004; 25: 4817-24.
  • 41. Matsumura K, Hyon SH, Nakajima N, Tsutsumi S. Effects on gingival cells of hydroxyapatite immobilized on poly(ethylene-co-vinyl alcohol). J Biomed Mater Res A 2007; 82: 288-95.
  • 42. Fillipi A. The influence of ozonised water on the epithelial wound healing process in the oral cavity. Radiology and Oral Medicine, 2001.
There are 42 citations in total.

Details

Subjects Health Care Administration
Journal Section Surgical Science Research Articles
Authors

Aysun Akpınar

Metin Çalışır

Ömer Poyraz

Fahrettin Göze

Derya Özdemir Doğan

Vildan Bostancı

Publication Date September 19, 2017
Acceptance Date August 22, 2017
Published in Issue Year 2017 Volume: 39 Issue: 3

Cite

AMA Akpınar A, Çalışır M, Poyraz Ö, Göze F, Özdemir Doğan D, Bostancı V. The effects of ozone on the local and systemic interleukin 1β (IL-1β) and IL-10 levels experimental periodontitis model in rats. CMJ. September 2017;39(3):608-619. doi:10.7197/223.v39i31705.347461

Cited By

The Effect of Ozone Gas on IL-1β and IL-10 Levels of Gingival Crevicular Fluid in Aggressive Periodontitis Patients
Ozone: Science & Engineering
Metin Çalışır
https://doi.org/10.1080/01919512.2019.1575183
Platelet rich fibrin versus ozone gel for periodontal regeneration in induced rats' intrabony three-wall periodontal defects
Journal of Oral Biology and Craniofacial Research
Aya Anwar Alsherif
https://doi.org/10.1016/j.jobcr.2020.09.001