The effect of boric acid on penicillin induced experimental epilepsy

Mustafa Karademir; Gökhan Arslan

doi:10.7197/223.vi.543145

TR EN

Borik asidin penisilin ile indüklenen deneysel epilepsi üzerine etkisi

Öz

Amaç: Günlük hayatta insanlar, bir bor bileşiği olan borik aside maruz kalmaktadırlar. Bu çalışmada, düşük dozlarda uygulanan borik asidin penisilin kaynaklı epileptiform aktivite üzerindeki etkisini araştırdık. Ayrıca, borik asidin gabapentinin antikonvülsan etkisindeki rolünü de inceledik.

Yöntem: Bu çalışmada, kırk sekiz adet erkek Wistar sıçanı kullanıldı. Üretan anestezisinden sonra, sıçanlar stereotaksi aletine bağlandı. Elektrokortikografi kayıtları için sol somatomotor korteks üzerine bir bipolar elektrot sabitlendi. İlk deney setinde, intrakortikal penisilin (500 IU) enjeksiyonundan 30 dakika sonra, dört farklı borik asit dozu (5, 10, 20 ve 40 mg/kg) intraperitoneal olarak uygulandı. İkinci deney setinde, borik asit (20 mg / kg), gabapentin (200 mg/kg) ve borik asit (20 mg/kg) + gabapentin (200 mg/kg) kombinasyonu uygulandı. İlaç enjeksiyonlarından sonra 120 dakika boyunca elektrokortikografi kayıtları kaydedildi ve sonrasında spike frekans ve amplitüdleri hesaplandı.

Bulgular: 5 mg/kg dozunda uygulanan borik asit, kontrol grubuyla karşılaştırıldığında spike frekansını anlamlı ölçüde değiştirmedi. 10, 20 ve 40 mg / kg'lık dozlarında uygulanan borik asit ise, sırasıyla 50., 30. ve 30. dakikalarda ortalama spike frekansını artırarak prokonvülsan aktivite gösterdi. Gabapentin 30. dakikadan sonra ortalama spike frekansını azalttı. 20 mg/kg dozunda uygulanan borik asit, gabapentinin antikonvülsan etkisini inhibe etti. Spike amplüdü açısından hiçbir grup arasında anlamlı fark bulunmadı.

Sonuç: Bu çalışma, borik asidin düşük dozlarının penisilin kaynaklı nöbetleri arttırdığını göstermektedir. Borik asit, bu prokonvulzan etkisini muhtemelen GABAerjik sistem üzerinden göstermektedir.

Anahtar Kelimeler

Epilepsi,Epileptiform aktivite,Penisilin,Gabapentin,Borik asit,Gabapentin

The effect of boric acid on penicillin induced experimental epilepsy

Abstract

Objective: In daily life, people are exposed to a boron compound, boric acid. This study was designed to investigate the effect of different low doses of boric acid on penicillin-induced epileptiform activity. The role of boric acid in the anticonvulsant effect of gabapentin was also researched.

Method: Forty-eight Male Wistar rats were used in this study. After uretan anesthesia, rats were attached to a stereotaxic device. A bipolar electrode was fixed over the left somatomotor cortex for electrocorticography (ECoG) recordings. In the first set of experiments, 30 min after intracortical injection of penicillin (500 IU), four different doses of boric acid were received (5, 10, 20 and 40 mg/kg) intraperitoneally (i.p.). In the second set of experiments, boric acid (20 mg/kg), gabapentin (200 mg/kg) and boric acid (20 mg/kg) + gabapentin (200 mg/kg) combination were administered. Electrocorticography recordings were persisted for 120 minutes after the drug injections and spike frequencies and amplitudes were calculated.

Results: Boric acid, at a dose of 5 mg/kg, did not significantly change the means of spike frequency when compared to the control group. At the doses of 10, 20 and 40 mg/kg, boric acid showed proconvulsant activity by increasing the mean spike frequency in the 50, 30 and 30 minutes, respectively. Gabapentin decreased the means of spike frequency in 30 min. Boric acid (20 mg/kg) inhibited the anticonvulsant effect of gabapentin. No significant difference detected between any groups in terms of spike amplitude.

Conclusions: The results of the present study show that low doses of boric acid increase penicillin-induced seizures. We suggested that boric acid may show its proconvulsant effect probably via GABAergic pathway.

Keywords

Epilepsy,Epileptiform activity,Penicillin,Boric acid,Gabapentin

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