Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)

Hüsnü Genç; Sinan Soylu; Deniz Şahin İnan; Atilla Kurt; Hakkı Çoşkun; Ali Yıldırır

doi:10.7197/cmj.904376

EN

Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)

Abstract

Objective: The basic aim in the treatment of second-degree burns is to prevent progressive cell death, and so treatments are directed at the zone of stasis. In this study, We were aimed to investigate the healing effects of using Mesenchymal Stem Cells (MSCs), Ovarian follicular fluid (OFS) and Platelet-rich plasma (PRP) on burn stasis zone in an experimental burn model.

Method: Forty rats were divided randomly into four groups. Burns were created in each group according to the comb burn model. The control group received no treatment; the mesenchymal stem cell [MSC] group received MSC; the platelet-rich plasma [PRP] group received PRP; and the ovarian follicular fluid [OFF] group received OFF subcutaneously on days 1, 3 and 5. On days 1 and 21, all rats were photographed and the burn sites were calculated. At the end of day 21, all rats were sacrificed, the dorsum containing the created burns were excised, and the epithelialization, collagen amount, fibroblast density, inflammatory cell density and VEGF amounts were evaluated histopathologically.

Results: The groups were assessed based on the burn site healing rate and histopathological scoring. Healing was faster in the MSC group [p<0.005], PRP group [p>0.005] and OFS group [p<0.005] than in the control group. When the treatment groups were compared with each other, the best healing was observed in the MSC, PRP and OFF groups, respectively.

Conclusions: MSC, PRP and OFF were found to have a positive effect on burn healing, with MSC being the most efficient method among the three, followed by PRP and OFF, respectively, which were found to provide a faster healing than the control group.

Keywords

Supporting Institution

CUBAP

Project Number

T-758

References

Refarans1 Pruitt, B. A., Wolf, S. E., & Mason, A. D. [2012]. Epidemiological, demographic, and outcome characteristics of burn injury. Total burn care, 4, 15-45.
Refarans2. Jackson, D. M. [1953]. The diagnosis of the depth of burning. British journal of surgery, 40[164], 588-596.
Refarans3. Shupp, J. W., Nasabzadeh, T. J., Rosenthal, D. S., Jordan, M. H., Fidler, P., & Jeng, J. C. [2010]. A review of the local pathophysiologic bases of burn wound progression. Journal of burn care & research, 31[6], 849-873.
Refarans4. Tan, J. Q., Zhang, H. H., Lei, Z. J., Ren, P., Deng, C., Li, X. Y., & Chen, S. Z. [2013]. The roles of autophagy and apoptosis in burn wound progression in rats. Burns, 39[8], 1551-1556.
Refarans5. Zawacki, B. E. [1974]. Reversal of capillary stasis and prevention of necrosis in burns. Annals of surgery, 180[1], 98.
Refarans6. Rangatchew, F., Vester-Glowinski, P., Rasmussen, B. S., Haastrup, E., Munthe-Fog, L., Talman, M. L., at all.. (2020). Mesenchymal stem cell therapy of acute thermal burns: A systematic review of the effect on inflammation and wound healing. Burns.Lacci, K. M., & Dardik, A. [2010]. Platelet-rich plasma: support for its use in wound healing. The Yale journal of biology and medicine, 83[1], 1.
Refarans7. Hammadeh, M. E., Ertan, A. K., Zeppezauer, M., Baltes, S., Georg, T., Rosenbaum, P., Schmidt, W. [2002]. Immunoglobulins and cytokines level in follicular fluid in relation to etiology of infertility and their relevance to IVF outcome. American Journal of Reproductive Immunology, 47[2], 82-90.
Refarans8. Ozornek, M. H., Bielfeld, P., Krüssel, J. S., Hirchenhain, J., Jeyendran, R. S., Koldovsky, U. [1999]. Epidermal growth factor and leukemia inhibitory factor levels in follicular fluid. Association with in vitro fertilization outcome. The Journal of reproductive medicine, 44[4], 367-369.

Refarans9. Mendoza, C., Ruiz-Requena, E., Ortega, E., Cremades, N., Martinez, F., Bernabeu, R., et al. [2002]. Follicular fluid markers of oocyte developmental potential. Human Reproduction, 17[4], 1017-1022.
Refarans10. Vujisic, S., Zidovec, S. [2005]. Follicular immunology environment and the influence on in vitro fertilization outcome. Current Women's Health Reviews, 1[1], 49-60.
Referans11. Calogero, A. E., Nicoletti, F., Palumbo, M. A., Burrello, N., Di Mauro, M., Lunetta, M., et al. [1998]. Macrophage-derived cytokines in the follikuler fluids of women infertility due to immunological causes. Elevated levels of interleukin 6 and low levels of granulocyte-macrophage colony-stimulating factor. Cytokine, 10[10], 814-818.
Referans12. Chae, H., Hong, S. H., Hong, S. H., Kim, S. H., Kim, C. H., Kang, B. M., et al. [2007]. Influence of tumor necrosis factor-α on estradiol, progesterone, insulin-like growth factor-II, and insulin-like growth factor binding protein-1, 2, and 3 in cultured human luteinized granulosa cells. European Journal of Obstetrics & Gynecology and Reproductive Biology, 131[2], 176-181.
Referans13. Pellicer, A., Albert, C., Mercader, A., Bonilla-Musoles, F., Remohı́, J., Simón, C. [1998]. The follicular and endocrine environment in women with endometriosis: local and systemic cytokine production. Fertility and sterility, 70[3], 425-431.
Referans14. Regas, F. C., Ehrlich, H. P. [1992]. Elucidating the vascular response to burns with a new rat model. The Journal of trauma, 32[5], 557-563.
Referans15. Pellicer, A., Albert, C., Mercader, A., Bonilla-Musoles, F., Remohí, J., Simón, C. [1999]. The pathogenesis of ovarian hyperstimulation syndrome: in vivo studies investigating the role of interleukin-1β, interleukin-6, and vascular endothelial growth factor. Fertility and sterility, 71[3], 482-489.
Referans16. Sharma, V. P., O'Boyle, C. P., Jeffery, S. L. [2011]. Man or machine? The clinimetric properties of laser Doppler imaging in burn depth assessment. Journal of Burn Care & Research, 32[1], 143-149.
Referans17. Sparkes, B. G. [1997]. Immunological responses to thermal injury. Burns, 23[2], 106-113.
Referans18. Nagato, H., Umebayashi, Y., Wako, M., Tabata, Y., Manabe, M. [2006]. Collagen–poly glycolic acid hybrid matrix with basic fibroblast growth factor accelerated angiogenesis and granulation tissue formation in diabetic mice. The Journal of dermatology, 33[10], 670-675.
Referans19. Nursal T.Z., Baykal A., Hamaloğlu E. Wound Healing in the Elderly: Is there a difference? Turkish Journal of Geriatrics Geriatri, 2[1]: 2932, [1999].
Referans20. Rodgers, K., Jadhav, S. S. [2018]. The application of mesenchymal stem cells to treat thermal and radiation burns. Advanced drug delivery reviews, 123, 75-81.
Referans21. Verstappen, J., Katsaros, C., Torensma, R., Von den Hoff, J. W. [2009]. A functional model for adult stem cells in epithelial tissues. Wound repair and regeneration, 17[3], 296-305.
Referans22. Wu, Y., Chen, L., Scott, P. G., Tredget, E. E. [2007]. Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem cells, 25[10], 2648-2659.
Referans23. . Zuk, P. A., Zhu, M. I. N., Mizuno, H., Huang, J., Futrell, J. W., Katz, A. J., et al. [2001]. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue engineering, 7[2], 211-228..
Referans24. Shumakov, V. I., Onishchenko, N. A., Rasulov, M. F., Krasheninnikov, M. E., & Zaidenov, V. A. [2003]. Mesenchymal bone marrow stem cells more effectively stimulate regeneration of deep burn wounds than embryonic fibroblasts. Bulletin of experimental biology and medicine, 136[2], 192-195.
Referans25. Falanga, V., Iwamoto, S., Chartier, M., Yufit, T., Butmarc, J., Kouttab, N., et al. [2007]. Autologous bone marrow–derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue engineering, 13[6], 1299-1312.
Referans26. Lu, D., Chen, B., Liang, Z., Deng, W., Jiang, Y., Li, S., et al. [2011]. Comparison of bone marrow mesenchymal stem cells with bone marrow-derived mononuclear cells for treatment of diabetic critical limb ischemia and foot ulcer: a double-blind, randomized, controlled trial. Diabetes research and clinical practice, 92[1], 26-36.
Referans27. Volarevic, V., Arsenijevic, N., Lukic, M. L., Stojkovic, M. [2011]. Concise review: mesenchymal stem cell treatment of the complications of diabetes mellitus. Stem cells, 29[1], 5-10.
Referans28. Lataillade, J. J., Doucet, C., Bey, E., Carsin, H., Huet, C., Clairand, I., et al. [2007]. New approach to radiation burn treatment by dosimetry-guided surgery combined with autologous mesenchymal stem cell therapy.
Referans29. Vojtaššák, J., Danišovič, L., Kubeš, M., Bakoš, D., Jarabek, L., Uličná, M., et al. [2006]. Autologous biograft and mesenchymal stem cells in treatment of the diabetic foot. Neuroendocrinology Letters, 27[supplement 2], 134-137.
Referans30. Nurden, A. T., Nurden, P., Sanchez, M., Andia, I., Anitua, E. [2008]. Platelets and wound healing. Frontiers in bioscience: a journal and virtual library, 13, 3532-3548.
Referans31. Peavy, G. M., Jacobson, M. W., Salmon, D. P., Gamst, A. C., Patterson, T. L., Goldman, S., et al. [2012]. The influence of chronic stress on dementia-related diagnostic change in older adults. Alzheimer disease and associated disorders, 26[3], 260..
Referans32. Su, C. Y., Kuo, Y. P., Nieh, H. L., Tseng, Y. H., Burnouf, T. [2008]. Quantitative assessment of the kinetics of growth factors release from platelet gel. Transfusion, 48[11], 2414-2420.
Referans33. Zimmermann, R., Arnold, D., Strasser, E., Ringwald, J., Schlegel, A., Wiltfang, J., et al. [2003]. Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates. Vox sanguinis, 85[4], 283-289.
Referans34. Tschon, M., Fini, M., Giardino, R., Filardo, G., Dallari, D., Torricelli, P., et al. [2011]. [Frontiers in Bioscience E3, 96-107, January 1, 2011] Lights and shadows concerning platelet products for musculoskeletal regeneration. Frontiers in Bioscience, 3, 96-107.
Referans35. Mazzucco, L., Borzini, P., Gope, R. [2010]. Platelet-derived factors involved in tissue repair—from signal to function. Transfusion medicine reviews, 24[3], 218-234. 105[S 06], S13-S33.
Referans36. Nurden, A. T. [2011]. Platelets, inflammation and tissue regeneration. Thrombosis and haemostasis, 105[S 06], S13-S33.
Referans37. Henderson, J. L., Cupp, C. L., Ross, E. V., Shick, P. C., Keefe, M. A., Wester, D. C., et al. [2003]. The effects of autologous platelet gel on wound healing. Ear, nose & throat journal, 82[8], 598-602.
Referans38. Kazakos, K., Lyras, D. N., Verettas, D., Tilkeridis, K., & Tryfonidis, M. [2009]. The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury, 40[8], 801-805.
Referans39. Liu, J., Qu, W., Li, R., Zheng, C., Zhang, L. [2018]. Efficacy of autologous platelet-rich gel in the treatment of deep grade II burn wounds. Int J Clin Exp Med, 11[3], 2654-2659.
Referans40. Singer, A. J., Toussaint, J., Chung, W. T., McClain, S., Raut, V., Rosenberg, L. [2018]. The effects of platelet rich plasma on healing of full thickness burns in swine. Burns, 44[6], 1543-1550.
Referans41. Marck, R. E., Middelkoop, E., & Breederveld, R. S. [2014]. Considerations on the use of platelet-rich plasma, specifically for burn treatment. Journal of Burn Care & Research, 35[3], 219-227.
Referans42. Moncayo HE, Penz-Koza A, Marth C, Gastl G, Herold M,Moncayo R. Vascular endothelial growth factor in serum and in the follicular fluid of patients undergoing hormonal stimulation for invitro fertilization: Hum Reprod 1998; 13[12]: 3310-4.
Referans43. Ayhan S. [2007]. Primer yara iyileşmesi üzerine embriyonik kök hücre ve ovaryum folikül sıvısının etkisi, Yayınlanmamış Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi, Eskişehir.

Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Hüsnü Genç
0000-0001-9514-7096
Türkiye

Sinan Soylu ^*
0000-0002-3911-3227
Türkiye

Deniz Şahin İnan
0000-0002-0292-4448
Türkiye

Atilla Kurt
0000-0002-3649-6293
Türkiye

Hakkı Çoşkun
0000-0001-7913-8325
Türkiye

Ali Yıldırır
0000-0001-8228-2862
Türkiye

Publication Date

March 31, 2021

Submission Date

March 27, 2021

Acceptance Date

March 31, 2021

Published in Issue

Year 1970 Volume: 43 Number: 1

DOI

https://doi.org/10.7197/cmj.904376

IZ

https://izlik.org/JA93FM97KH

Cite

RIS / Bibtex

AMA

1.Genç H, Soylu S, Şahin İnan D, Kurt A, Çoşkun H, Yıldırır A. Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study). CMJ. 2021;43(1):62-71. doi:10.7197/cmj.904376

Öz

Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)

Abstract

Keywords

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite