Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)

Hüsnü Genç; Sinan Soylu; Deniz Şahin İnan; Atilla Kurt; Hakkı Çoşkun; Ali Yıldırır

doi:10.7197/cmj.904376

EN

Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)

Abstract

Objective: The basic aim in the treatment of second-degree burns is to prevent progressive cell death, and so treatments are directed at the zone of stasis. In this study, We were aimed to investigate the healing effects of using Mesenchymal Stem Cells (MSCs), Ovarian follicular fluid (OFS) and Platelet-rich plasma (PRP) on burn stasis zone in an experimental burn model.

Method: Forty rats were divided randomly into four groups. Burns were created in each group according to the comb burn model. The control group received no treatment; the mesenchymal stem cell [MSC] group received MSC; the platelet-rich plasma [PRP] group received PRP; and the ovarian follicular fluid [OFF] group received OFF subcutaneously on days 1, 3 and 5. On days 1 and 21, all rats were photographed and the burn sites were calculated. At the end of day 21, all rats were sacrificed, the dorsum containing the created burns were excised, and the epithelialization, collagen amount, fibroblast density, inflammatory cell density and VEGF amounts were evaluated histopathologically.

Results: The groups were assessed based on the burn site healing rate and histopathological scoring. Healing was faster in the MSC group [p<0.005], PRP group [p>0.005] and OFS group [p<0.005] than in the control group. When the treatment groups were compared with each other, the best healing was observed in the MSC, PRP and OFF groups, respectively.

Conclusions: MSC, PRP and OFF were found to have a positive effect on burn healing, with MSC being the most efficient method among the three, followed by PRP and OFF, respectively, which were found to provide a faster healing than the control group.

Keywords

Destekleyen Kurum

CUBAP

Proje Numarası

T-758

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Hüsnü Genç
0000-0001-9514-7096
Türkiye

Sinan Soylu ^*
0000-0002-3911-3227
Türkiye

Deniz Şahin İnan
0000-0002-0292-4448
Türkiye

Atilla Kurt
0000-0002-3649-6293
Türkiye

Hakkı Çoşkun
0000-0001-7913-8325
Türkiye

Ali Yıldırır
0000-0001-8228-2862
Türkiye

Yayımlanma Tarihi

31 Mart 2021

Gönderilme Tarihi

27 Mart 2021

Kabul Tarihi

31 Mart 2021

Yayımlandığı Sayı

Yıl 1970 Cilt: 43 Sayı: 1

DOI

https://doi.org/10.7197/cmj.904376

IZ

https://izlik.org/JA93FM97KH

Kaynak Göster

RIS / Bibtex

AMA

1.Genç H, Soylu S, Şahin İnan D, Kurt A, Çoşkun H, Yıldırır A. Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study). CMJ. 2021;43(1):62-71. doi:10.7197/cmj.904376

Öz

Comparison of the effect of stem cell, platelete rich plasma and ovarium folicular fluid on burn stasis zone (Experimental study)

Abstract

Keywords

Destekleyen Kurum

Proje Numarası

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster