Plasmid DNA isolation and characterization studies

Murat Doğan

doi:10.7197/cmj.987190

EN

Plasmid DNA isolation and characterization studies

Abstract

Objective: The use of gene therapy for targeted therapy in various genetic diseases has become more and more popular day by day. Various carrier vectors are used in gene therapy. The plasmid we are interested in in this study is used as a gene delivery vector. In this study, it was aimed to perform the isolation and characterization studies of the peGFP-N1 plasmid. In addition, necessary properties were investigated to make it a suitable gene carrier vector.

Method: In this study, isolation was performed using the plasmid peGFP-N1 via classical method. Escherichia coli was used as expression medium in the isolation process. In order to calculate the amount of plasmid DNA in the studies, the spectral curve value of the plasmid DNA was calculated. Spectrophotometric methods were used to determine the amount and impurity of the isolated bacterial DNA.

Results: In plasmid DNA isolation studies, it was observed that the yield was quite high and the contamination from protein or RNA was very low. In fact, the ratio of absorbance values (A1/A2) is very close to 1.8 as a result of the isolation III study shows that there is no contamination.

Conclusions: The isolation and spectrophotometric study results of the peGFP-N1 plasmid showed that this plasmid is suitable and usable in terms of impurity and contamination risk. By making use of the results of this study, it will support the studies in which this plasmid can be used in an appropriate gene carrier system.

Keywords

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Murat Doğan ^*
0000-0003-2794-0177
Türkiye

Publication Date

September 30, 2021

Submission Date

August 25, 2021

Acceptance Date

September 25, 2021

Published in Issue

Year 2021 Volume: 43 Number: 3

DOI

https://doi.org/10.7197/cmj.987190

IZ

https://izlik.org/JA94SA62ZG

Cite

RIS / Bibtex

APA

Doğan, M. (2021). Plasmid DNA isolation and characterization studies. Cumhuriyet Medical Journal, 43(3), 226-231. https://doi.org/10.7197/cmj.987190

AMA

1.Doğan M. Plasmid DNA isolation and characterization studies. CMJ. 2021;43(3):226-231. doi:10.7197/cmj.987190

Chicago

Doğan, Murat. 2021. “Plasmid DNA Isolation and Characterization Studies”. Cumhuriyet Medical Journal 43 (3): 226-31. https://doi.org/10.7197/cmj.987190.

EndNote

Doğan M (September 1, 2021) Plasmid DNA isolation and characterization studies. Cumhuriyet Medical Journal 43 3 226–231.

IEEE

[1]M. Doğan, “Plasmid DNA isolation and characterization studies”, CMJ, vol. 43, no. 3, pp. 226–231, Sept. 2021, doi: 10.7197/cmj.987190.

ISNAD

Doğan, Murat. “Plasmid DNA Isolation and Characterization Studies”. Cumhuriyet Medical Journal 43/3 (September 1, 2021): 226-231. https://doi.org/10.7197/cmj.987190.

JAMA

1.Doğan M. Plasmid DNA isolation and characterization studies. CMJ. 2021;43:226–231.

MLA

Doğan, Murat. “Plasmid DNA Isolation and Characterization Studies”. Cumhuriyet Medical Journal, vol. 43, no. 3, Sept. 2021, pp. 226-31, doi:10.7197/cmj.987190.

Vancouver

1.Murat Doğan. Plasmid DNA isolation and characterization studies. CMJ. 2021 Sep. 1;43(3):226-31. doi:10.7197/cmj.987190