Plasmid DNA isolation and characterization studies

Murat Doğan

doi:10.7197/cmj.987190

EN

Plasmid DNA isolation and characterization studies

Öz

Objective: The use of gene therapy for targeted therapy in various genetic diseases has become more and more popular day by day. Various carrier vectors are used in gene therapy. The plasmid we are interested in in this study is used as a gene delivery vector. In this study, it was aimed to perform the isolation and characterization studies of the peGFP-N1 plasmid. In addition, necessary properties were investigated to make it a suitable gene carrier vector.

Method: In this study, isolation was performed using the plasmid peGFP-N1 via classical method. Escherichia coli was used as expression medium in the isolation process. In order to calculate the amount of plasmid DNA in the studies, the spectral curve value of the plasmid DNA was calculated. Spectrophotometric methods were used to determine the amount and impurity of the isolated bacterial DNA.

Results: In plasmid DNA isolation studies, it was observed that the yield was quite high and the contamination from protein or RNA was very low. In fact, the ratio of absorbance values (A1/A2) is very close to 1.8 as a result of the isolation III study shows that there is no contamination.

Conclusions: The isolation and spectrophotometric study results of the peGFP-N1 plasmid showed that this plasmid is suitable and usable in terms of impurity and contamination risk. By making use of the results of this study, it will support the studies in which this plasmid can be used in an appropriate gene carrier system.

Anahtar Kelimeler

Kaynakça

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6. Klindworth A, Pruesse E, Schweer T. et al. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next- generation sequencing-based diversity studies. Nucleic Acids Res. 2013; 41: e1. doi: 10.1093/nar/gks808.
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Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Murat Doğan ^*
0000-0003-2794-0177
Türkiye

Yayımlanma Tarihi

30 Eylül 2021

Gönderilme Tarihi

25 Ağustos 2021

Kabul Tarihi

25 Eylül 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 43 Sayı: 3

DOI

https://doi.org/10.7197/cmj.987190

IZ

https://izlik.org/JA94SA62ZG

Kaynak Göster

RIS / Bibtex

APA

Doğan, M. (2021). Plasmid DNA isolation and characterization studies. Cumhuriyet Medical Journal, 43(3), 226-231. https://doi.org/10.7197/cmj.987190

AMA

1.Doğan M. Plasmid DNA isolation and characterization studies. CMJ. 2021;43(3):226-231. doi:10.7197/cmj.987190

Chicago

Doğan, Murat. 2021. “Plasmid DNA isolation and characterization studies”. Cumhuriyet Medical Journal 43 (3): 226-31. https://doi.org/10.7197/cmj.987190.

EndNote

Doğan M (01 Eylül 2021) Plasmid DNA isolation and characterization studies. Cumhuriyet Medical Journal 43 3 226–231.

IEEE

[1]M. Doğan, “Plasmid DNA isolation and characterization studies”, CMJ, c. 43, sy 3, ss. 226–231, Eyl. 2021, doi: 10.7197/cmj.987190.

ISNAD

Doğan, Murat. “Plasmid DNA isolation and characterization studies”. Cumhuriyet Medical Journal 43/3 (01 Eylül 2021): 226-231. https://doi.org/10.7197/cmj.987190.

JAMA

1.Doğan M. Plasmid DNA isolation and characterization studies. CMJ. 2021;43:226–231.

MLA

Doğan, Murat. “Plasmid DNA isolation and characterization studies”. Cumhuriyet Medical Journal, c. 43, sy 3, Eylül 2021, ss. 226-31, doi:10.7197/cmj.987190.

Vancouver

1.Murat Doğan. Plasmid DNA isolation and characterization studies. CMJ. 01 Eylül 2021;43(3):226-31. doi:10.7197/cmj.987190