Research Article
BibTex RIS Cite

Year 2019, , 676 - 684, 31.12.2019

Turabi Güneş

https://doi.org/10.7197/cmj.vi.584608

Abstract

Project Number

SHMYO-OO5

References

  • 1. Shapiro ED, Gerber MA. Lyme disease. Clin Infect Dis. 2000; 31(2):533–42.
  • 2. Stanek G, Wormser GP, Gray J, Strle F. Lyme borreliosis. Lancet (London, England). 2012;379(9814):461–73.
  • 3. Parola P, Paddock CD, Socolovschi C, Labruna MB, Mediannikov O, Kernif T, et al. Update on tick-borne rickettsioses around the world: a geographic approach. Clin Microbiol Rev. 2013;26(4):657–702.
  • 4. Carriveau A, Poole H, Thomas A. Lyme Disease. Nurs Clin North Am. 2019;54(2):261–75.
  • 5. Wilske B, Fingerle V, Schulte-Spechtel U. Microbiological and serological diagnosis of Lyme borreliosis. FEMS Immunol Med Microbiol. 2007;49(1):13–21.
  • 6. Buckingham SC. Tick-borne infections in children: epidemiology, clinical manifestations, and optimal management strategies. Paediatr Drugs. 2005;7(3):163–76.
  • 7. Gunes T, Kaya S, Poyraz O, Engin A. The prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in the Sinop region of Turkey. Turkish J Vet Anim Sci. 2007;31(3):153-158.
  • 8. Poyraz O, Gunes T. [Seroprevalance of Babesia microti in humans living in rural areas of the Sinop region]. Turkish Society for Parasitology. 2010;34(2):81-85.
  • 9. Gunes T, Poyraz O, Atas M, Turgut NH. The seroprevalence of Anaplasma phagocytophilum in humans from two different climatic regions of Turkey and its co-seroprevalence rate with Borrelia burgdorferi. Turkish J Med Sci. 2011;41(5):903-908.
  • 10. Gunes T, Poyraz O, Atas M, Alim A. Seroprevalence of tick-borne encephalitis virus (TBEV) among the residents of rural areas in Sinop, central black-sea region, Turkey. Mikrobiyol Bul. 2010;44(4):585-91.
  • 11. Vural T, Ergin C, Sayin F. Investigation of Rickettsia conorii antibodies in the Antalya area. Infection. 1998;26(3):170–2.
  • 12. Kuloglu F, Rolain JM, Aydoslu B, Akata F, Tugrul M, Raoult D. Prospective evaluation of rickettsioses in the Trakya (European) region of Turkey and atypic presentations of Rickettsia conorii. Ann N Y Acad Sci. 2006;1078(1):173–5.
  • 13. Kaya AD, Parlak AH, Ozturk CE, Behcet M. Seroprevalence of Borrelia burgdorferi infection among forestry workers and farmers in Duzce, north-western Turkey. New Microbiol. 2008;31(2):203–9.
  • 14. Rad AY, Hekimler K. Seroepidemiologic Study On Lyme Borreliosis In Isparta Region In Turkey. Hacettepe J Biol Chem. 2007;35(2):105–9.
  • 15. Punda-Polić V, Klismanić Z, Capkun V. Prevalence of antibodies to spotted fever group rickettsiae in the region of Split (southern Croatia). Eur J Epidemiol. 2003;18(5):451–5.
  • 16. Daniel SA, Manika K, Arvanmdou M, Antoniadis A. Prevalence of Rickettsia conorii and Rickettsia typhi infections in the population of northern Greece. Am J Trop Med Hyg. 2002 Jan 1;66(1):76–9.
  • 17. Gunes T, Poyraz O, Ataş M, Turgut NH. The seroprevalence of Rickettsia conorii in humans living in villages of Tokat Province in Turkey, where Crimean-Congo hemorrhagic fever virus is endemic, and epidemiological similarities of both infectious agents. Turkish J Med Sci. 2012;42(3):441-48.
  • 18. Hvidsten D, Mortensen L, Straume B, Arsenovic MG, Pedersen A-B, Lyngås G, et al. Blood donor Borrelia burgdorferi sensu lato seroprevalence and history of tick bites at a northern limit of the vector distribution. APMIS. 2017;125(8):717–24.
  • 19. Hristea A, Hristescu S, Ciufecu C, Vasile A. Seroprevalence of Borrelia burgdorferi in Romania. Eur J Epidemiol. 2001;17(9):891–6.
  • 20. Zając V, Pinkas J, Wójcik-Fatla A, Dutkiewicz J, Owoc A, Bojar I. Prevalence of serological response to Borrelia burgdorferi in farmers from eastern and central Poland. Eur J Clin Microbiol Infect Dis [Internet]. 2017;36(3):437–46.
  • 21. Akar N. Seroprevalence of hantavirus and Borrelia burgdorferi in Düzce (Turkey) forest villages and the relationship with sociodemographic features. Turkish J Med Sci. 2019;49(2):483–9.
  • 22. Alakus I, Sahin Horasan E, Kurt AO, Evik G, Ersoz G, Aslan G, et al. Seroprevalence of rickettsioses among healthy individuals in Mersin province, Turkey Turk J Public Health. 2016;14(3):128–134.
  • 23. Kalish RA, McHugh G, Granquist J, Shea B, Ruthazer R, Steere AC. Persistence of immunoglobulin M or immunoglobulin G antibody responses to Borrelia burgdorferi 10-20 years after active Lyme disease. Clin Infect Dis. 2001;33(6):780–5.
  • 24. Mansueto S, Vitale G, Bentivegna M, Tringali G, Di Leo R. Persistence of antibodies to Rickettsia conorii after an acute attack of boutonneuse fever. J Infect Dis. 1985;151(2):377.
  • 25. Sood SK, Salzman MB, Johnson BJ, Happ CM, Feig K, Carmody L, et al. Duration of tick attachment as a predictor of the risk of Lyme disease in an area in which Lyme disease is endemic. J Infect Dis. 1997;175(4):996–9.
  • 26. Saraiva DG, Soares HS, Soares JF, Labruna MB. Feeding Period Required by Amblyomma aureolatum Ticks for Transmission of Rickettsia rickettsii to Vertebrate Hosts. Emerg Infect Dis. 2014;20(9):1504–10.
  • 27. Brouqui P, Parola P, Fournier PE, Raoult D. Spotted fever rickettsioses in southern and eastern Europe. FEMS Immunol Med Microbiol. 2007;49(1):2–12.
  • 28. Karagun E, Ozcan Y. Early Stage Lyme Disease Diagnosis: Two Case Reports. Turkiye Klin J Dermatology. 2018;29(1):36–9.
  • 29. Kuloglu F, Rolain JM, Fournier PE, Akata F, Tugrul M, Raoult D. First isolation of Rickettsia conorii from humans in the Trakya (European) region of Turkey. Eur J Clin Microbiol Infect Dis. 2004;23(8):609–14.

The investigation of seroprevalence of Borrelia burgdorferi and Rickettsia conorii in people living in forest villages of Sinop

Year 2019, , 676 - 684, 31.12.2019

Turabi Güneş

https://doi.org/10.7197/cmj.vi.584608

Abstract

Objective: This study aimed to
investigate the seroprevalence of Borrelia
burgdorferi, the causative agent of Lyme disease, and Rickettsia conorii, the causative agent of Mediterranean spotted
fever, in the Sinop region.

Method: In 2006 and 2007, the blood
serum of 184 people, living in the villages of the central district of Sinop,
was obtained and stored at 80ºC until the study. In these sera, IgG antibodies reactive to B. burgdorferi and R. conorii were investigated by ELISA method.

Results: Antibody positivity was found against B.
burgdorferi in 36.41% and R. conorii
in 45.10% of 184 people living in rural areas of Sinop. Reactive antibodies against B. burgdorferi were detected in 47.62% of 42 people with a history
of redness and swelling at the contact site after the tick bite, and in 28.95%
of 76 people without these symptoms (p= 0.043, OR=2.23). The seropositivity to R. conorii was found in 57.14% and 46.05%
of persons with and without these symptoms, respectively (p= 0.363, OR= 1.56).
There was a statistically significant increase
in seroprevalence of both B. burgdorferi
and R. conorii with increasing age (p<0.05). R. conorii and
B. burgdorferi co-seroprevalence were
determined in 16.85% of serum samples. Since the ELISA test results in both pathogens were not repeated with
confirmation tests, these findings were considered possible.







Conclusions: The findings obtained in this study revealed that
Sinop and similar ecological features should be evaluated within the endemic
regions for B. burgdorferi and R. conorii. It should be paying attention that infections
from both pathogens may be likely to appear at these regions.

Keywords

Borrelia burgdorferi Rickettsia conorii Lyme disease Mediterranean Spotted Fever Erythema Migrans

Supporting Institution

The Cumhuriyet University Scientific Research Projects Commission Presidentship

Project Number

SHMYO-OO5

Thanks

This study was supported by the Cumhuriyet University Scientific Research Projects Commission Presidentship (CUBAP) (SHMYO-OO5). During the investigation, I would like to thank the High Nurse Zübeyde Güneş for helping to collect blood from people, and thanks for Prof. Dr Ömer Poyraz and Prof. Dr Ahmet Alim for their contributions in ELISA and RPR testing.

References

  • 1. Shapiro ED, Gerber MA. Lyme disease. Clin Infect Dis. 2000; 31(2):533–42.
  • 2. Stanek G, Wormser GP, Gray J, Strle F. Lyme borreliosis. Lancet (London, England). 2012;379(9814):461–73.
  • 3. Parola P, Paddock CD, Socolovschi C, Labruna MB, Mediannikov O, Kernif T, et al. Update on tick-borne rickettsioses around the world: a geographic approach. Clin Microbiol Rev. 2013;26(4):657–702.
  • 4. Carriveau A, Poole H, Thomas A. Lyme Disease. Nurs Clin North Am. 2019;54(2):261–75.
  • 5. Wilske B, Fingerle V, Schulte-Spechtel U. Microbiological and serological diagnosis of Lyme borreliosis. FEMS Immunol Med Microbiol. 2007;49(1):13–21.
  • 6. Buckingham SC. Tick-borne infections in children: epidemiology, clinical manifestations, and optimal management strategies. Paediatr Drugs. 2005;7(3):163–76.
  • 7. Gunes T, Kaya S, Poyraz O, Engin A. The prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in the Sinop region of Turkey. Turkish J Vet Anim Sci. 2007;31(3):153-158.
  • 8. Poyraz O, Gunes T. [Seroprevalance of Babesia microti in humans living in rural areas of the Sinop region]. Turkish Society for Parasitology. 2010;34(2):81-85.
  • 9. Gunes T, Poyraz O, Atas M, Turgut NH. The seroprevalence of Anaplasma phagocytophilum in humans from two different climatic regions of Turkey and its co-seroprevalence rate with Borrelia burgdorferi. Turkish J Med Sci. 2011;41(5):903-908.
  • 10. Gunes T, Poyraz O, Atas M, Alim A. Seroprevalence of tick-borne encephalitis virus (TBEV) among the residents of rural areas in Sinop, central black-sea region, Turkey. Mikrobiyol Bul. 2010;44(4):585-91.
  • 11. Vural T, Ergin C, Sayin F. Investigation of Rickettsia conorii antibodies in the Antalya area. Infection. 1998;26(3):170–2.
  • 12. Kuloglu F, Rolain JM, Aydoslu B, Akata F, Tugrul M, Raoult D. Prospective evaluation of rickettsioses in the Trakya (European) region of Turkey and atypic presentations of Rickettsia conorii. Ann N Y Acad Sci. 2006;1078(1):173–5.
  • 13. Kaya AD, Parlak AH, Ozturk CE, Behcet M. Seroprevalence of Borrelia burgdorferi infection among forestry workers and farmers in Duzce, north-western Turkey. New Microbiol. 2008;31(2):203–9.
  • 14. Rad AY, Hekimler K. Seroepidemiologic Study On Lyme Borreliosis In Isparta Region In Turkey. Hacettepe J Biol Chem. 2007;35(2):105–9.
  • 15. Punda-Polić V, Klismanić Z, Capkun V. Prevalence of antibodies to spotted fever group rickettsiae in the region of Split (southern Croatia). Eur J Epidemiol. 2003;18(5):451–5.
  • 16. Daniel SA, Manika K, Arvanmdou M, Antoniadis A. Prevalence of Rickettsia conorii and Rickettsia typhi infections in the population of northern Greece. Am J Trop Med Hyg. 2002 Jan 1;66(1):76–9.
  • 17. Gunes T, Poyraz O, Ataş M, Turgut NH. The seroprevalence of Rickettsia conorii in humans living in villages of Tokat Province in Turkey, where Crimean-Congo hemorrhagic fever virus is endemic, and epidemiological similarities of both infectious agents. Turkish J Med Sci. 2012;42(3):441-48.
  • 18. Hvidsten D, Mortensen L, Straume B, Arsenovic MG, Pedersen A-B, Lyngås G, et al. Blood donor Borrelia burgdorferi sensu lato seroprevalence and history of tick bites at a northern limit of the vector distribution. APMIS. 2017;125(8):717–24.
  • 19. Hristea A, Hristescu S, Ciufecu C, Vasile A. Seroprevalence of Borrelia burgdorferi in Romania. Eur J Epidemiol. 2001;17(9):891–6.
  • 20. Zając V, Pinkas J, Wójcik-Fatla A, Dutkiewicz J, Owoc A, Bojar I. Prevalence of serological response to Borrelia burgdorferi in farmers from eastern and central Poland. Eur J Clin Microbiol Infect Dis [Internet]. 2017;36(3):437–46.
  • 21. Akar N. Seroprevalence of hantavirus and Borrelia burgdorferi in Düzce (Turkey) forest villages and the relationship with sociodemographic features. Turkish J Med Sci. 2019;49(2):483–9.
  • 22. Alakus I, Sahin Horasan E, Kurt AO, Evik G, Ersoz G, Aslan G, et al. Seroprevalence of rickettsioses among healthy individuals in Mersin province, Turkey Turk J Public Health. 2016;14(3):128–134.
  • 23. Kalish RA, McHugh G, Granquist J, Shea B, Ruthazer R, Steere AC. Persistence of immunoglobulin M or immunoglobulin G antibody responses to Borrelia burgdorferi 10-20 years after active Lyme disease. Clin Infect Dis. 2001;33(6):780–5.
  • 24. Mansueto S, Vitale G, Bentivegna M, Tringali G, Di Leo R. Persistence of antibodies to Rickettsia conorii after an acute attack of boutonneuse fever. J Infect Dis. 1985;151(2):377.
  • 25. Sood SK, Salzman MB, Johnson BJ, Happ CM, Feig K, Carmody L, et al. Duration of tick attachment as a predictor of the risk of Lyme disease in an area in which Lyme disease is endemic. J Infect Dis. 1997;175(4):996–9.
  • 26. Saraiva DG, Soares HS, Soares JF, Labruna MB. Feeding Period Required by Amblyomma aureolatum Ticks for Transmission of Rickettsia rickettsii to Vertebrate Hosts. Emerg Infect Dis. 2014;20(9):1504–10.
  • 27. Brouqui P, Parola P, Fournier PE, Raoult D. Spotted fever rickettsioses in southern and eastern Europe. FEMS Immunol Med Microbiol. 2007;49(1):2–12.
  • 28. Karagun E, Ozcan Y. Early Stage Lyme Disease Diagnosis: Two Case Reports. Turkiye Klin J Dermatology. 2018;29(1):36–9.
  • 29. Kuloglu F, Rolain JM, Fournier PE, Akata F, Tugrul M, Raoult D. First isolation of Rickettsia conorii from humans in the Trakya (European) region of Turkey. Eur J Clin Microbiol Infect Dis. 2004;23(8):609–14.
There are 29 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Basic Science Research Articles
Authors

Turabi Güneş 0000-0002-8011-7844

Project Number SHMYO-OO5
Publication Date December 31, 2019
Acceptance Date December 31, 2019
Published in Issue Year 2019

Cite

AMA Güneş T. The investigation of seroprevalence of Borrelia burgdorferi and Rickettsia conorii in people living in forest villages of Sinop. CMJ. December 2019;41(4):676-684. doi:10.7197/cmj.vi.584608