Cumhuriyet Medical Journal
Research Article
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Hemostatic Effects of A-fact, which is a new hemostatic agent

Year 2018, Volume 40, Issue 4, 326 - 333, 29.12.2018

Ceylan HEPOKUR Sema MISIR Mahmut ÖZBEK

https://doi.org/10.7197/223.vi.440027
Cited By: 1

Abstract

Background: The use of hemostatic agents is used to stop the bleeding. In medicine, an ideal hemostatic agent should have been stop venous bleeding after being applied to the lesion. This study investigated the in vitro effects of A-Fact a hemostatic parameter. Methods: It has been determined in human plasma at different rates. Hemostatic parameters were determined using routine biochemical methods. For morphological evaluation in full blood, microscopic images of 5%, 10%, 15% and 50% drugs applied to blood samples were taken. Results: A-Fact induced the very fast formation of protein network. Coagulation test was performed on 2/20 dilution. The coagulation factor (II, VII, IX) was studied. The levels of coagulation factors were not affected by A-Fact. Plasma fibrinogen activities, total protein, albumin and globulin levels decreased, in parallel and with the prolonged prothrombin time. And also INR values were increased following the addition of the A-Fact. Conclusion: Our results suggested that the mechanism of A-Fact bleeding stopper has shown the formation of the protein network. These data support that A-Fact may be evaluated as a new hemostatic agent.Background: The use of hemostatic agents is used to stop the bleeding. In medicine, an ideal hemostatic agent should have been stop venous bleeding after being applied to the lesion. This study investigated the in vitro effects of A-Fact a hemostatic parameter. Methods: It has been determined in human plasma at different rates. Hemostatic parameters were determined using routine biochemical methods. For morphological evaluation in full blood, microscopic images of 5%, 10%, 15% and 50% drugs applied to blood samples were taken. Results: A-Fact induced the very fast formation of protein network. Coagulation test was performed on 2/20 dilution. The coagulation factor (II, VII, IX) was studied. The levels of coagulation factors were not affected by A-Fact. Plasma fibrinogen activities, total protein, albumin and globulin levels decreased, in parallel and with the prolonged prothrombin time. And also INR values were increased following the addition of the A-Fact. Conclusion: Our results suggested that the mechanism of A-Fact bleeding stopper has shown the formation of the protein network. These data support that A-Fact may be evaluated as a new hemostatic agent. 

 

Keywords

A-Fact, Blood Stopper, Hematology

References

  • 1. Ferhanoğlu B. Hemostaz Mekanizması. Hematoloji Ders Kitabı. ISBN: 978-975-404-815-5.Üniversite Yayın No: 4774.Cerrahpaşa Tıp Fak. Yayın No: 269:Istanbul, 2008,p.219- 227.2. Colman RW, Hırsh J, Moider VJ. Hemostasis and Trombosis, Basic Principles and Clinical Practise.6th ed.USA: Lippincott Williams,2004,p.381-400.
  • 3. Carmona RH, Lim RC, Clark GC. Morbidity and mortality in hepatic trauma. Am J Surg 1982;144:88-94.
  • 4. Kaynaroğlu ZV. Karın travmaları. Ed:Sayeki. Temel cerrahi.3th ed. Ankara: Güneş kitabevi. 2004,466-472.
  • 5. Parks RW, Chrysos E, Diamond T. Management of liver trauma. Br J Surg 1999; 86: 1121-1135.
  • 6. Secer HI, Daneyemez M, Tehli O, Gonul E, Izci Y. The clinical, electrophysiologic, and surgical characteristics of peripheral nerve injuries caused by gunshot wounds in adults: a 40-year experience. SurgNeurol 2008;69:143-52.
  • 7. Sönmez E, Çavuş UY, Civelek C, Dur A, Karayel E, Gülen B, Uysal Ö, İpek G. The efficacy of a hemostatic agent in anticoagulant drug-induced rat bleeding model. Ulus Travma Acil Cerr Derg March 2014; 20: 2.
  • 8. Kurata M, Horii I. Blood coagulation tests in toxicological studies review of methods and their significance for drug safety assesment. J.Toxical Sci 2004;29:13-32.
  • 9. Daniel NG, Goulet J, Bergeron M, Paquin R, Landry Pe. Antitrombosit Drugs:ıs there a surgical risk? J can dent Assoc 2002;68:683-687.
  • Degroot Pg, Sixma Jj. Trombosit Adhesion. Br J haematol 1990; 75:308-312.
  • Goker H, Haznedaroglu IC, Ercetın S, Kırazlı S, Akman U, Ozturk Y, Hc Fırat HC. Haemostatic Actions of the Folkloric Medicinal Plant Extract Ankaferd Blood Stopper. The Journal of International Medical Research 2008; 36:163-170.
  • Seyednejad H, Imani M, Jamieson T, Seifalian AM. Topical haemostatic agents. British Journal of Surgery 2008; 95: 1197-1225.
  • 13. Pogorielov MV, Sikora VZ. Chitosan as a Hemostatic Agent: Current State. European Journal of Medicine. Series B, 2015, Vol.(2), Is. 1.
  • 14. Lapierre F, Houtaud SD, Wager M, Hemostatic Agents in Neurosurgery. Explicative Cases of Controversial Issues in Neurosurger 2011,doi:10.5772/31319.
  • Uzun N, Tanriverdi T, Savrun FK, Kiziltan ME, Sahin R, Hanimoglu H, et al. Traumatic peripheral nerve injuries: demographic and electrophysiologic findings of 802 patients from a developing country. J ClinNeuromuscul Dis 2006;7:97-103
  • 16. Vance GN and Etheresia P. Iron-enhanced coagulation is attenuated by chelation:a thrombelastographic and ultrastructural analysis. Blood Coagulation and Fibrinolysis 2014;25:845–850.
  • 17. Jankun J, Landeta P, Pretorıus E, Skrzypczak-Jankun E, Lıpınskı B. Unusual clotting dynamics of plasmasupplemented with iron(III). Internatıonal Journal Of Molecular Medıcıne 2014; 33: 367-372.
  • Clark WR, Leather RP, Hemostasis during liver resections. Surgery 1970;67:5556-5576.
  • Evans BE. Local hemostatic agents. N.Y. J. Dent 1977; 47: 109-14.
  • Bleeding Control and Healing Aid Compositions and Methods of Use, Terence Prevendar, US 6,652,840 B1, Nov. 25, 2003.
  • 21. Taylor CA, Braza D, Rice JB, Dillingham T. The incidence of peripheral nerve injury in extremity trauma. Am J Phys Med Rehabil 2008;87:381-5.
  • Al B, Yildirim C, Cavdar M, Zengin S, Buyukaslan H, Kalender ME. Effectiveness of Ankaferd blood stopper in the topical control of active bleeding due to cutaneous-subcutaneous incisions. Saudi Med J 2009; 30: 1520–5.
  • 23. Vance GN, Etheresia P. Iron-enhanced coagulation is attenuated by chelation:a thrombelastographic and ultrastructural analysis. Blood Coagulation and Fibrinolysis 2014;25:845–50.
  • Aysan E, Bektas H, Ersoz F, Sari S, Kaygusuz A, Huq GE. Ability of the ankaferd blood stopper® to prevent parenchymal bleeding in an experimental hepatic trauma model. Int J Clin Exp Med 2010; 3: 186–91. 2.
  • Beyazit Y, Kurt M, Kekilli M, Goker H, Haznedaroglu IC. Evaluation of hemostatic effects of Ankaferd as an alternative medicine. Altern Med Rev 2010; 15: 329–36. 3.
  • Beyazit Y, Kekilli M, Haznedaroglu IC, Kayacetin E, Basaranoglu M. Ankaferd hemostat in the management of gastrointestinal hemorrhages. World J Gastroenterol 2011; 17: 3962–70. 4.
  • 27. Tasdelen Fisgin N, Tanriverdi Cayci Y, Coban AY, Ozatli D, Tanyel E, Durupinar B, Tulek N. Antimicrobial activity of plant extract Ankaferd Blood Stopper. Fitoterapia 2009; 80: 48–50. 5.
  • Kandemir O, Buyukates M, Kandemir NO, Aktunc E, Gul AE, Gul S, Turan SA. Demonstration of the histopathological and immunohistochemical effects of a novel hemostatic agent, Ankaferd Blood Stopper, on vascular tissue in a rat aortic bleeding model. J Cardiothorac Surg 2010; 5: 110. 6.
  • Yüce S, Çandirli C, Yenidünya S, Muslu B. New hemostatic agent: the effect of Ankaferd Blood Stopper on healing wounds in experimental skin incision model. Turk J Med Sci 2014; 44: 288-294.

Yeni bir hemostatik ajan olan A-Fact’ın hemostatik etkileri

Year 2018, Volume 40, Issue 4, 326 - 333, 29.12.2018

Ceylan HEPOKUR Sema MISIR Mahmut ÖZBEK

https://doi.org/10.7197/223.vi.440027
Cited By: 1

Abstract

Amaç: Kanamayı durdurmak için hemostatik ajanların kullanılır. Tıpta, ideal bir hemostatik ajan lezyona uygulandıktan sonra venöz kanamayı durdurmalıdır. Bu çalışmada A-Fact’ın hemostatik parametrelerinin invitro etkileri araştırılacaktır. Yöntem: İnsan plazmasında farklı oranlarda belirlenmiştir. Hemostatik parametreler rutin biyokimyasal yöntemler kullanılarak belirlendi. Tam kanda morfolojik değerlendirme için kan örneklerine% 5,% 10,% 15 ve% 50 ilaçların mikroskobik görüntüleri alındı. Bulgular: A-Fact, protein ağının çok hızlı oluşumunu sağladı. Koagülasyon testi 2/20 seyreltme üzerinde yapıldı. Koagülasyon faktörü 2,7 ve 9 çalışıldı ve A-Fact koagülasyon faktörlerini etkilemedi. . Plazma fibrinojen aktivitesi, total protein, albümin ve globulin düzeyleri azalırken buna paralel olarak protrombin zamanı uzadı. Ayrıca, A-Fact eklendikten sonra INR değerleri artırıldı. Sonuç: Sonuçlarımıza göre; A-Fact kanama durdurucunun mekanizması protein ağı oluşumunu gösterdi. Elde edilen veriler A-fact’ı yeni bir hemostatik ajan olarak gösterdi.

Keywords

Anahtar Kelimeler: A-Fact, Hematoloji, Hemostatik Ajan

References

  • 1. Ferhanoğlu B. Hemostaz Mekanizması. Hematoloji Ders Kitabı. ISBN: 978-975-404-815-5.Üniversite Yayın No: 4774.Cerrahpaşa Tıp Fak. Yayın No: 269:Istanbul, 2008,p.219- 227.2. Colman RW, Hırsh J, Moider VJ. Hemostasis and Trombosis, Basic Principles and Clinical Practise.6th ed.USA: Lippincott Williams,2004,p.381-400.
  • 3. Carmona RH, Lim RC, Clark GC. Morbidity and mortality in hepatic trauma. Am J Surg 1982;144:88-94.
  • 4. Kaynaroğlu ZV. Karın travmaları. Ed:Sayeki. Temel cerrahi.3th ed. Ankara: Güneş kitabevi. 2004,466-472.
  • 5. Parks RW, Chrysos E, Diamond T. Management of liver trauma. Br J Surg 1999; 86: 1121-1135.
  • 6. Secer HI, Daneyemez M, Tehli O, Gonul E, Izci Y. The clinical, electrophysiologic, and surgical characteristics of peripheral nerve injuries caused by gunshot wounds in adults: a 40-year experience. SurgNeurol 2008;69:143-52.
  • 7. Sönmez E, Çavuş UY, Civelek C, Dur A, Karayel E, Gülen B, Uysal Ö, İpek G. The efficacy of a hemostatic agent in anticoagulant drug-induced rat bleeding model. Ulus Travma Acil Cerr Derg March 2014; 20: 2.
  • 8. Kurata M, Horii I. Blood coagulation tests in toxicological studies review of methods and their significance for drug safety assesment. J.Toxical Sci 2004;29:13-32.
  • 9. Daniel NG, Goulet J, Bergeron M, Paquin R, Landry Pe. Antitrombosit Drugs:ıs there a surgical risk? J can dent Assoc 2002;68:683-687.
  • Degroot Pg, Sixma Jj. Trombosit Adhesion. Br J haematol 1990; 75:308-312.
  • Goker H, Haznedaroglu IC, Ercetın S, Kırazlı S, Akman U, Ozturk Y, Hc Fırat HC. Haemostatic Actions of the Folkloric Medicinal Plant Extract Ankaferd Blood Stopper. The Journal of International Medical Research 2008; 36:163-170.
  • Seyednejad H, Imani M, Jamieson T, Seifalian AM. Topical haemostatic agents. British Journal of Surgery 2008; 95: 1197-1225.
  • 13. Pogorielov MV, Sikora VZ. Chitosan as a Hemostatic Agent: Current State. European Journal of Medicine. Series B, 2015, Vol.(2), Is. 1.
  • 14. Lapierre F, Houtaud SD, Wager M, Hemostatic Agents in Neurosurgery. Explicative Cases of Controversial Issues in Neurosurger 2011,doi:10.5772/31319.
  • Uzun N, Tanriverdi T, Savrun FK, Kiziltan ME, Sahin R, Hanimoglu H, et al. Traumatic peripheral nerve injuries: demographic and electrophysiologic findings of 802 patients from a developing country. J ClinNeuromuscul Dis 2006;7:97-103
  • 16. Vance GN and Etheresia P. Iron-enhanced coagulation is attenuated by chelation:a thrombelastographic and ultrastructural analysis. Blood Coagulation and Fibrinolysis 2014;25:845–850.
  • 17. Jankun J, Landeta P, Pretorıus E, Skrzypczak-Jankun E, Lıpınskı B. Unusual clotting dynamics of plasmasupplemented with iron(III). Internatıonal Journal Of Molecular Medıcıne 2014; 33: 367-372.
  • Clark WR, Leather RP, Hemostasis during liver resections. Surgery 1970;67:5556-5576.
  • Evans BE. Local hemostatic agents. N.Y. J. Dent 1977; 47: 109-14.
  • Bleeding Control and Healing Aid Compositions and Methods of Use, Terence Prevendar, US 6,652,840 B1, Nov. 25, 2003.
  • 21. Taylor CA, Braza D, Rice JB, Dillingham T. The incidence of peripheral nerve injury in extremity trauma. Am J Phys Med Rehabil 2008;87:381-5.
  • Al B, Yildirim C, Cavdar M, Zengin S, Buyukaslan H, Kalender ME. Effectiveness of Ankaferd blood stopper in the topical control of active bleeding due to cutaneous-subcutaneous incisions. Saudi Med J 2009; 30: 1520–5.
  • 23. Vance GN, Etheresia P. Iron-enhanced coagulation is attenuated by chelation:a thrombelastographic and ultrastructural analysis. Blood Coagulation and Fibrinolysis 2014;25:845–50.
  • Aysan E, Bektas H, Ersoz F, Sari S, Kaygusuz A, Huq GE. Ability of the ankaferd blood stopper® to prevent parenchymal bleeding in an experimental hepatic trauma model. Int J Clin Exp Med 2010; 3: 186–91. 2.
  • Beyazit Y, Kurt M, Kekilli M, Goker H, Haznedaroglu IC. Evaluation of hemostatic effects of Ankaferd as an alternative medicine. Altern Med Rev 2010; 15: 329–36. 3.
  • Beyazit Y, Kekilli M, Haznedaroglu IC, Kayacetin E, Basaranoglu M. Ankaferd hemostat in the management of gastrointestinal hemorrhages. World J Gastroenterol 2011; 17: 3962–70. 4.
  • 27. Tasdelen Fisgin N, Tanriverdi Cayci Y, Coban AY, Ozatli D, Tanyel E, Durupinar B, Tulek N. Antimicrobial activity of plant extract Ankaferd Blood Stopper. Fitoterapia 2009; 80: 48–50. 5.
  • Kandemir O, Buyukates M, Kandemir NO, Aktunc E, Gul AE, Gul S, Turan SA. Demonstration of the histopathological and immunohistochemical effects of a novel hemostatic agent, Ankaferd Blood Stopper, on vascular tissue in a rat aortic bleeding model. J Cardiothorac Surg 2010; 5: 110. 6.
  • Yüce S, Çandirli C, Yenidünya S, Muslu B. New hemostatic agent: the effect of Ankaferd Blood Stopper on healing wounds in experimental skin incision model. Turk J Med Sci 2014; 44: 288-294.

Details

Primary Language English
Subjects Health Care Sciences and Services
Published Date Aralık 2018
Journal Section Basic Science Research Articles
Authors

Ceylan HEPOKUR>

Türkiye

Sema MISIR>

Türkiye

Mahmut ÖZBEK This is me

Publication Date December 29, 2018
Published in Issue Year 2018, Volume 40Issue 4

Cite

APA Hepokur, C. , Mısır, S. & Özbek, M. (2018). Hemostatic Effects of A-fact, which is a new hemostatic agent . Cumhuriyet Medical Journal , 40 (4) , 326-333 . DOI: 10.7197/223.vi.440027

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