The investigation of seroprevalence of Borrelia burgdorferi and Rickettsia conorii in people living in forest villages of Sinop

Turabi Güneş

doi:10.7197/cmj.vi.584608

EN

The investigation of seroprevalence of Borrelia burgdorferi and Rickettsia conorii in people living in forest villages of Sinop

Abstract

Objective: This study aimed to investigate the seroprevalence of Borrelia burgdorferi, the causative agent of Lyme disease, and Rickettsia conorii, the causative agent of Mediterranean spotted fever, in the Sinop region.

Method: In 2006 and 2007, the blood serum of 184 people, living in the villages of the central district of Sinop, was obtained and stored at 80ºC until the study. In these sera, IgG antibodies reactive to B. burgdorferi and R. conorii were investigated by ELISA method.

Results: Antibody positivity was found against B. burgdorferi in 36.41% and R. conorii in 45.10% of 184 people living in rural areas of Sinop. Reactive antibodies against B. burgdorferi were detected in 47.62% of 42 people with a history of redness and swelling at the contact site after the tick bite, and in 28.95% of 76 people without these symptoms (p= 0.043, OR=2.23). The seropositivity to R. conorii was found in 57.14% and 46.05% of persons with and without these symptoms, respectively (p= 0.363, OR= 1.56). There was a statistically significant increase in seroprevalence of both B. burgdorferi and R. conorii with increasing age (p<0.05). R. conorii and B. burgdorferi co-seroprevalence were determined in 16.85% of serum samples. Since the ELISA test results in both pathogens were not repeated with confirmation tests, these findings were considered possible.

Conclusions: The findings obtained in this study revealed that Sinop and similar ecological features should be evaluated within the endemic regions for B. burgdorferi and R. conorii. It should be paying attention that infections from both pathogens may be likely to appear at these regions.

Keywords

Borrelia burgdorferi,Rickettsia conorii,Lyme disease,Mediterranean Spotted Fever,Erythema Migrans

Destekleyen Kurum

The Cumhuriyet University Scientific Research Projects Commission Presidentship

Proje Numarası

SHMYO-OO5

Teşekkür

This study was supported by the Cumhuriyet University Scientific Research Projects Commission Presidentship (CUBAP) (SHMYO-OO5). During the investigation, I would like to thank the High Nurse Zübeyde Güneş for helping to collect blood from people, and thanks for Prof. Dr Ömer Poyraz and Prof. Dr Ahmet Alim for their contributions in ELISA and RPR testing.

Kaynakça

1. Shapiro ED, Gerber MA. Lyme disease. Clin Infect Dis. 2000; 31(2):533–42.
2. Stanek G, Wormser GP, Gray J, Strle F. Lyme borreliosis. Lancet (London, England). 2012;379(9814):461–73.
3. Parola P, Paddock CD, Socolovschi C, Labruna MB, Mediannikov O, Kernif T, et al. Update on tick-borne rickettsioses around the world: a geographic approach. Clin Microbiol Rev. 2013;26(4):657–702.
4. Carriveau A, Poole H, Thomas A. Lyme Disease. Nurs Clin North Am. 2019;54(2):261–75.
5. Wilske B, Fingerle V, Schulte-Spechtel U. Microbiological and serological diagnosis of Lyme borreliosis. FEMS Immunol Med Microbiol. 2007;49(1):13–21.
6. Buckingham SC. Tick-borne infections in children: epidemiology, clinical manifestations, and optimal management strategies. Paediatr Drugs. 2005;7(3):163–76.
7. Gunes T, Kaya S, Poyraz O, Engin A. The prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in the Sinop region of Turkey. Turkish J Vet Anim Sci. 2007;31(3):153-158.
8. Poyraz O, Gunes T. [Seroprevalance of Babesia microti in humans living in rural areas of the Sinop region]. Turkish Society for Parasitology. 2010;34(2):81-85.

9. Gunes T, Poyraz O, Atas M, Turgut NH. The seroprevalence of Anaplasma phagocytophilum in humans from two different climatic regions of Turkey and its co-seroprevalence rate with Borrelia burgdorferi. Turkish J Med Sci. 2011;41(5):903-908.
10. Gunes T, Poyraz O, Atas M, Alim A. Seroprevalence of tick-borne encephalitis virus (TBEV) among the residents of rural areas in Sinop, central black-sea region, Turkey. Mikrobiyol Bul. 2010;44(4):585-91.
11. Vural T, Ergin C, Sayin F. Investigation of Rickettsia conorii antibodies in the Antalya area. Infection. 1998;26(3):170–2.
12. Kuloglu F, Rolain JM, Aydoslu B, Akata F, Tugrul M, Raoult D. Prospective evaluation of rickettsioses in the Trakya (European) region of Turkey and atypic presentations of Rickettsia conorii. Ann N Y Acad Sci. 2006;1078(1):173–5.
13. Kaya AD, Parlak AH, Ozturk CE, Behcet M. Seroprevalence of Borrelia burgdorferi infection among forestry workers and farmers in Duzce, north-western Turkey. New Microbiol. 2008;31(2):203–9.
14. Rad AY, Hekimler K. Seroepidemiologic Study On Lyme Borreliosis In Isparta Region In Turkey. Hacettepe J Biol Chem. 2007;35(2):105–9.
15. Punda-Polić V, Klismanić Z, Capkun V. Prevalence of antibodies to spotted fever group rickettsiae in the region of Split (southern Croatia). Eur J Epidemiol. 2003;18(5):451–5.
16. Daniel SA, Manika K, Arvanmdou M, Antoniadis A. Prevalence of Rickettsia conorii and Rickettsia typhi infections in the population of northern Greece. Am J Trop Med Hyg. 2002 Jan 1;66(1):76–9.
17. Gunes T, Poyraz O, Ataş M, Turgut NH. The seroprevalence of Rickettsia conorii in humans living in villages of Tokat Province in Turkey, where Crimean-Congo hemorrhagic fever virus is endemic, and epidemiological similarities of both infectious agents. Turkish J Med Sci. 2012;42(3):441-48.
18. Hvidsten D, Mortensen L, Straume B, Arsenovic MG, Pedersen A-B, Lyngås G, et al. Blood donor Borrelia burgdorferi sensu lato seroprevalence and history of tick bites at a northern limit of the vector distribution. APMIS. 2017;125(8):717–24.
19. Hristea A, Hristescu S, Ciufecu C, Vasile A. Seroprevalence of Borrelia burgdorferi in Romania. Eur J Epidemiol. 2001;17(9):891–6.
20. Zając V, Pinkas J, Wójcik-Fatla A, Dutkiewicz J, Owoc A, Bojar I. Prevalence of serological response to Borrelia burgdorferi in farmers from eastern and central Poland. Eur J Clin Microbiol Infect Dis [Internet]. 2017;36(3):437–46.
21. Akar N. Seroprevalence of hantavirus and Borrelia burgdorferi in Düzce (Turkey) forest villages and the relationship with sociodemographic features. Turkish J Med Sci. 2019;49(2):483–9.
22. Alakus I, Sahin Horasan E, Kurt AO, Evik G, Ersoz G, Aslan G, et al. Seroprevalence of rickettsioses among healthy individuals in Mersin province, Turkey Turk J Public Health. 2016;14(3):128–134.
23. Kalish RA, McHugh G, Granquist J, Shea B, Ruthazer R, Steere AC. Persistence of immunoglobulin M or immunoglobulin G antibody responses to Borrelia burgdorferi 10-20 years after active Lyme disease. Clin Infect Dis. 2001;33(6):780–5.
24. Mansueto S, Vitale G, Bentivegna M, Tringali G, Di Leo R. Persistence of antibodies to Rickettsia conorii after an acute attack of boutonneuse fever. J Infect Dis. 1985;151(2):377.
25. Sood SK, Salzman MB, Johnson BJ, Happ CM, Feig K, Carmody L, et al. Duration of tick attachment as a predictor of the risk of Lyme disease in an area in which Lyme disease is endemic. J Infect Dis. 1997;175(4):996–9.
26. Saraiva DG, Soares HS, Soares JF, Labruna MB. Feeding Period Required by Amblyomma aureolatum Ticks for Transmission of Rickettsia rickettsii to Vertebrate Hosts. Emerg Infect Dis. 2014;20(9):1504–10.
27. Brouqui P, Parola P, Fournier PE, Raoult D. Spotted fever rickettsioses in southern and eastern Europe. FEMS Immunol Med Microbiol. 2007;49(1):2–12.
28. Karagun E, Ozcan Y. Early Stage Lyme Disease Diagnosis: Two Case Reports. Turkiye Klin J Dermatology. 2018;29(1):36–9.
29. Kuloglu F, Rolain JM, Fournier PE, Akata F, Tugrul M, Raoult D. First isolation of Rickettsia conorii from humans in the Trakya (European) region of Turkey. Eur J Clin Microbiol Infect Dis. 2004;23(8):609–14.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Turabi Güneş ^*
0000-0002-8011-7844
Türkiye

Yayımlanma Tarihi

31 Aralık 2019

Gönderilme Tarihi

30 Haziran 2019

Kabul Tarihi

31 Aralık 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 41 Sayı: 4

DOI

https://doi.org/10.7197/cmj.vi.584608

IZ

https://izlik.org/JA92CZ72XF

Kaynak Göster

RIS / Bibtex

AMA

1.Güneş T. The investigation of seroprevalence of Borrelia burgdorferi and Rickettsia conorii in people living in forest villages of Sinop. CMJ. 2019;41(4):676-684. doi:10.7197/cmj.vi.584608

Öz

The investigation of seroprevalence of Borrelia burgdorferi and Rickettsia conorii in people living in forest villages of Sinop

Abstract

Keywords

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster