Abstract
Özet
Amaç. Çalışmamızda şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etkisini tespit etmek amaçlanmıştır. Yöntem. Erzincan Bahçe Bitkileri Araştırma Enstitüsü Gen Bahçesi’nde bulunan Cardinal çeşidi 7 adet şeftali (Persica vulgaris Miller) ağacı yaprağı methanol ekstraktı bu amaçla kullanılmıştır. Antibakteriyel etkisinin araştırılmasında cut plug yöntemi kullanılırken Minimum İnhibisyon Konsantrasyonu (MİK), mikrodilüsyon yöntemi ile belirlenmiştir. Bulgular. Bu çalışmada şeftali (Persica vulgaris Miller) yaprak ektraktının Staphylococcus aureus, Staphylococcus aureus (MRSA)’a karşı antibakteriyel etkisi belirlenemezken, Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus ,Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica bakterilerine karşı ise antibakteriyel etki tespit edilmiştir. En düşük Minimum İnhibisyon Konsantrasyonu Pseudomonas aeruginosa ve Yersinia enterocolitica’da tespit edilirken (0,01 mg/mL), en yüksek konsantrasyon Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes’de görülmüştür (10 mg/mL). Sonuç. Çalışmamız sonucunda şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etki spektrumu invitro olarak tespit edilmiş olup bu tür bitkilerden yola çıkılarak yeni antibakteriyel ajanların oluşum sürecinin başlatılabileceği düşünülmektedir.
Anahtar sözcükler: Persica vulgaris Miller, antibakteriyel etki, cut plug metot, methanol ekstraktı.
Abstract
Aim. In our study we aimed to determine antibacterial effect of peach leaves extract. Methods. In Erzincan Horticultural Research Institute Gen Garden varietes seven cardinal peach’s (Persica vulgaris Miller (Rosaceae) tree leaf methanol extract was used for this purpose, Antibacterial effect was evaluated by cutplug method whereas minimum inhibtory concentration (MIC) was determined by microdilution method. Results. In this study peach’s (Persica vulgaris Miller ) leaf extract antibacterial effect on Staphylococcus aureus, Staphylococcus aureus (MRSA) was not determined, on the other hand the antibacterial effects on Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica was determined. The lowest minimum inhibitory concentration on Pseudomonas aeruginosa and Yersinia enterocolitica detected as (0.01 mg/mL), the higest concentration on grup of Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes was determined as (10 mg/mL). Conclusions. As a result of our study peach’s (Persica vulgaris Miller) leaves extract spectrum showed broad antibacterial effect. Starting from such plants, thought to be started the process of the formation of new antibacterial agents.
Keywords: Persica vulgaris Miller, antibacteriel effect, cut plug methot, methanol extractReferences
- 1. Toroğlu S, Dığrak M, Çenet M. Baharat olarak tüketilen Laurus nobilis linn ve Zingiber officinale roscoe bitki uçucu yağlarının antimikrobiyal aktiviteleri ve antibiyotiklere in-vitro etkilerinin belirlenmesi” KSU. Fen ve Mühendislik Dergisi 2006; 9: 20-6.
- 2. Ilçım A, Dığrak M, Bitki Ekstraktlarının Antimikrobiyal Etkilerinin Araştırılması. Tr J Biol 1998; 22: 119-25.
- 3. Lin J, Opoku AR, Geheeb-Keller M, Hutchings AD, Terblanche E, Ja ger AK, Van Staden J. Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities”. J Ethnopharmacol 1999; 68: 267-74.
- 4. Pridham T, Lindenfelser L, Shotwell O, Stodola F, Benedict R, Foley C, Jacks P, Zaumeyer W, Perston W, Mitchell J: Antibiotics against plant diseases: A laboratory and green house survey. Phytopathology 1956; 46: 568-75.
- 5. C.L.S.I. Clinical Laboratory Standards Institute Performance Standards For Antimicrobial Disk And Dilution Susceptibility Tests For Bacteria Isolated From Animals; Approved Standard. Second Edition. M31-A2 and M37-A2. Clinical Laboratory Standards Institute Pennsylvania, USA, 2012.
- 6. N.C.C.L. National Committee for Clinical Laboratory Standards: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - Eighth edition. NCCLS document M7-A6. NCCLS, Wayne, Pennsylvania, USA, 2003.
- 7. Bilgehan H. Klinik Mikrobiyolojik Tanı, Barış Yayınları, Bornova, 1995; 283-5.
- 8. Kalayou S, Haileselassie M, Gebre-egziabher G, Tiku'e T, Sahle S, Taddele H, Ghezu M. In-vitro antimicrobial activity screening of some ethnoveterinary medicinal plants traditionally used against mastitis, wound and gastrointestinal tract complication in Tigray Region, Ethiopia. Asian Pac J Trop Biomed 2012; 2:516-22.
- 9. Murugan K, Sekar K, Sangeetha S, Ranjitha S, Sohaibani SA, Antibiofilm and quorum sensing inhibitory activity of Achyranthes aspera on cariogenic Streptococcus mutans: An in vitro and in silico study. Pharm Biol 2013; 51; 6:728-36.
- 10. Serpi M, Özdemir ZÖ, Salman Y. Bazı Bitki Ekstrelerinin Propionibacterium acnes Üzerine Antibakteriyel Etkilerinin Araştırılması. KSÜ Doğa Bil Derg 2012; 15: 7-12.
- 11. Duman Aydın B. Bazı Tıbbi Bitki ve Baharatların Gıda Patojenleri Üzerine Antibakteriyel Etkisinin Araştırılması. Kafkas Üniv Vet Fak Derg 2008; 14: 83-7.
- 12. Özkan O, Aydın H, Bağcıgil AF. Salvia verticillata ve Phlomis pungens’in in vitro antibakteriyel etkinliğinin değerlendirilmesi. Kafkas Univ Vet Fak Derg 2009; 15: 587-90.
Abstract
Aim. In our study we aimed to determine antibacterial effect of peach leaves extract. Methods. In Erzincan Horticultural Research Institute Gen Garden varietes seven cardinal peach’s (Persica vulgaris Miller (Rosaceae) tree leaf methanol extract was used for this purpose, Antibacterial effect was evaluated by cutplug method whereas minimum inhibtory concentration (MIC) was determined by microdilution method. Results. In this study peach’s (Persica vulgaris Miller ) leaf extract antibacterial effect on Staphylococcus aureus, Staphylococcus aureus (MRSA) was not determined, on the other hand the antibacterial effects on Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica was determined. The lowest minimum inhibitory concentration on Pseudomonas aeruginosa and Yersinia enterocolitica detected as (0.01 mg/mL), the higest concentration on grup of Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes was determined as (10 mg/mL). Conclusions. As a result of our study peach’s (Persica vulgaris Miller) leaves extract spectrum showed broad antibacterial effect. Starting from such plants, thought to be started the process of the formation of new antibacterial agents.
References
- 1. Toroğlu S, Dığrak M, Çenet M. Baharat olarak tüketilen Laurus nobilis linn ve Zingiber officinale roscoe bitki uçucu yağlarının antimikrobiyal aktiviteleri ve antibiyotiklere in-vitro etkilerinin belirlenmesi” KSU. Fen ve Mühendislik Dergisi 2006; 9: 20-6.
- 2. Ilçım A, Dığrak M, Bitki Ekstraktlarının Antimikrobiyal Etkilerinin Araştırılması. Tr J Biol 1998; 22: 119-25.
- 3. Lin J, Opoku AR, Geheeb-Keller M, Hutchings AD, Terblanche E, Ja ger AK, Van Staden J. Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities”. J Ethnopharmacol 1999; 68: 267-74.
- 4. Pridham T, Lindenfelser L, Shotwell O, Stodola F, Benedict R, Foley C, Jacks P, Zaumeyer W, Perston W, Mitchell J: Antibiotics against plant diseases: A laboratory and green house survey. Phytopathology 1956; 46: 568-75.
- 5. C.L.S.I. Clinical Laboratory Standards Institute Performance Standards For Antimicrobial Disk And Dilution Susceptibility Tests For Bacteria Isolated From Animals; Approved Standard. Second Edition. M31-A2 and M37-A2. Clinical Laboratory Standards Institute Pennsylvania, USA, 2012.
- 6. N.C.C.L. National Committee for Clinical Laboratory Standards: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - Eighth edition. NCCLS document M7-A6. NCCLS, Wayne, Pennsylvania, USA, 2003.
- 7. Bilgehan H. Klinik Mikrobiyolojik Tanı, Barış Yayınları, Bornova, 1995; 283-5.
- 8. Kalayou S, Haileselassie M, Gebre-egziabher G, Tiku'e T, Sahle S, Taddele H, Ghezu M. In-vitro antimicrobial activity screening of some ethnoveterinary medicinal plants traditionally used against mastitis, wound and gastrointestinal tract complication in Tigray Region, Ethiopia. Asian Pac J Trop Biomed 2012; 2:516-22.
- 9. Murugan K, Sekar K, Sangeetha S, Ranjitha S, Sohaibani SA, Antibiofilm and quorum sensing inhibitory activity of Achyranthes aspera on cariogenic Streptococcus mutans: An in vitro and in silico study. Pharm Biol 2013; 51; 6:728-36.
- 10. Serpi M, Özdemir ZÖ, Salman Y. Bazı Bitki Ekstrelerinin Propionibacterium acnes Üzerine Antibakteriyel Etkilerinin Araştırılması. KSÜ Doğa Bil Derg 2012; 15: 7-12.
- 11. Duman Aydın B. Bazı Tıbbi Bitki ve Baharatların Gıda Patojenleri Üzerine Antibakteriyel Etkisinin Araştırılması. Kafkas Üniv Vet Fak Derg 2008; 14: 83-7.
- 12. Özkan O, Aydın H, Bağcıgil AF. Salvia verticillata ve Phlomis pungens’in in vitro antibakteriyel etkinliğinin değerlendirilmesi. Kafkas Univ Vet Fak Derg 2009; 15: 587-90.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Basic Science Research Articles |
| Authors | |
| Publication Date | April 17, 2013 |
| Published in Issue | Year 2013Volume: 35 Issue: 2 |